Head-group conformation in phospholipids: a phosphorus-31 nuclear magnetic resonance study of oriented monodomain dipalmitoylphosphatidylcholine bilayers

R. G. Griffin, Linda S Powers, P. S. Pershan

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Angular-dependent 31P NMR spectra of oriented biaxial monodomain DPPC-H2O multilayers are employed to study head-group conformation in this phospholipid. The results indicate that the O-P-O plane of the phosphate, where the O's are the nonesterified oxygens of the phosphodiester, is tilted at 47 ± 5° with respect to the bilayer normal. This PO4 orientation could result in the choline moiety being extended parallel to the bilayer plane, and it will explain the breadth of the axially symmetric 31P powder spectrum observed for DPPC in excess water. This work is the first direct observation of this conformation for lecithins and it illustrates the utility of high-resolution solid-state NMR in structural studies of disordered systems.

Original languageEnglish (US)
Pages (from-to)2718-2722
Number of pages5
JournalBiochemistry
Volume17
Issue number14
StatePublished - 1978
Externally publishedYes

Fingerprint

1,2-Dipalmitoylphosphatidylcholine
Lecithins
Choline
Powders
Phosphorus
Conformations
Phospholipids
Magnetic Resonance Spectroscopy
Phosphates
Nuclear magnetic resonance
Observation
Oxygen
Water
Multilayers

ASJC Scopus subject areas

  • Biochemistry

Cite this

Head-group conformation in phospholipids : a phosphorus-31 nuclear magnetic resonance study of oriented monodomain dipalmitoylphosphatidylcholine bilayers. / Griffin, R. G.; Powers, Linda S; Pershan, P. S.

In: Biochemistry, Vol. 17, No. 14, 1978, p. 2718-2722.

Research output: Contribution to journalArticle

@article{4e188e2bdc4a4a8aa635492cf33ee5ea,
title = "Head-group conformation in phospholipids: a phosphorus-31 nuclear magnetic resonance study of oriented monodomain dipalmitoylphosphatidylcholine bilayers",
abstract = "Angular-dependent 31P NMR spectra of oriented biaxial monodomain DPPC-H2O multilayers are employed to study head-group conformation in this phospholipid. The results indicate that the O-P-O plane of the phosphate, where the O's are the nonesterified oxygens of the phosphodiester, is tilted at 47 ± 5° with respect to the bilayer normal. This PO4 orientation could result in the choline moiety being extended parallel to the bilayer plane, and it will explain the breadth of the axially symmetric 31P powder spectrum observed for DPPC in excess water. This work is the first direct observation of this conformation for lecithins and it illustrates the utility of high-resolution solid-state NMR in structural studies of disordered systems.",
author = "Griffin, {R. G.} and Powers, {Linda S} and Pershan, {P. S.}",
year = "1978",
language = "English (US)",
volume = "17",
pages = "2718--2722",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "14",

}

TY - JOUR

T1 - Head-group conformation in phospholipids

T2 - a phosphorus-31 nuclear magnetic resonance study of oriented monodomain dipalmitoylphosphatidylcholine bilayers

AU - Griffin, R. G.

AU - Powers, Linda S

AU - Pershan, P. S.

PY - 1978

Y1 - 1978

N2 - Angular-dependent 31P NMR spectra of oriented biaxial monodomain DPPC-H2O multilayers are employed to study head-group conformation in this phospholipid. The results indicate that the O-P-O plane of the phosphate, where the O's are the nonesterified oxygens of the phosphodiester, is tilted at 47 ± 5° with respect to the bilayer normal. This PO4 orientation could result in the choline moiety being extended parallel to the bilayer plane, and it will explain the breadth of the axially symmetric 31P powder spectrum observed for DPPC in excess water. This work is the first direct observation of this conformation for lecithins and it illustrates the utility of high-resolution solid-state NMR in structural studies of disordered systems.

AB - Angular-dependent 31P NMR spectra of oriented biaxial monodomain DPPC-H2O multilayers are employed to study head-group conformation in this phospholipid. The results indicate that the O-P-O plane of the phosphate, where the O's are the nonesterified oxygens of the phosphodiester, is tilted at 47 ± 5° with respect to the bilayer normal. This PO4 orientation could result in the choline moiety being extended parallel to the bilayer plane, and it will explain the breadth of the axially symmetric 31P powder spectrum observed for DPPC in excess water. This work is the first direct observation of this conformation for lecithins and it illustrates the utility of high-resolution solid-state NMR in structural studies of disordered systems.

UR - http://www.scopus.com/inward/record.url?scp=0017822501&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017822501&partnerID=8YFLogxK

M3 - Article

C2 - 581178

AN - SCOPUS:0017822501

VL - 17

SP - 2718

EP - 2722

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 14

ER -