Hemocompatibility of poly(vinyl alcohol)-gelatin core-shell electrospun nanofibers: A scaffold for modulating platelet deposition and activation

Valerie M. Merkle, Daniel Martin, Marcus Hutchinson, Phat L. Tran, Alana Behrens, Samir Hossainy, Jawaad Sheriff, Danny Bluestein, Xiaoyi Wu, Marvin J Slepian

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In this study, we evaluate coaxial electrospun nanofibers with gelatin in the shell and poly(vinyl alcohol) (PVA) in the core as a potential vascular material by determining fiber surface roughness, as well as human platelet deposition and activation under varying conditions. PVA scaffolds had the highest surface roughness (Ra = 65.5 ± 6.8 nm) but the lowest platelet deposition (34.2 ± 5.8 platelets) in comparison to gelatin nanofibers (Ra = 36.8 ± 3.0 nm and 168.9 ± 29.8 platelets) and coaxial nanofibers (1 Gel:1 PVA coaxial, Ra = 24.0 ± 1.5 nm and 150.2 ± 17.4 platelets. 3 Gel:1 PVA coaxial, Ra = 37.1 ± 2.8 nm and 167.8 ± 15.4 platelets). Therefore, the chemical structure of the gelatin nanofibers dominated surface roughness in platelet deposition. Due to their increased stiffness, the coaxial nanofibers had the highest platelet activation rate, rate of thrombin formation, in comparison to gelatin and PVA fibers. Our studies indicate that mechanical stiffness is a dominating factor for platelet deposition and activation, followed by biochemical signals, and lastly surface roughness. Overall, these coaxial nanofibers are an appealing material for vascular applications by supporting cellular growth while minimizing platelet deposition and activation.

Original languageEnglish (US)
Pages (from-to)8302-8312
Number of pages11
JournalACS Applied Materials and Interfaces
Volume7
Issue number15
DOIs
StatePublished - Apr 22 2015

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Gelatin
Nanofibers
Platelets
Scaffolds
Alcohols
Chemical activation
Surface roughness
Gels
Stiffness
Fibers
Thrombin

Keywords

  • Coaxial Electrospinning
  • Gelatin
  • Platelets
  • Poly(vinyl alcohol)
  • Tissue Engineering

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Hemocompatibility of poly(vinyl alcohol)-gelatin core-shell electrospun nanofibers : A scaffold for modulating platelet deposition and activation. / Merkle, Valerie M.; Martin, Daniel; Hutchinson, Marcus; Tran, Phat L.; Behrens, Alana; Hossainy, Samir; Sheriff, Jawaad; Bluestein, Danny; Wu, Xiaoyi; Slepian, Marvin J.

In: ACS Applied Materials and Interfaces, Vol. 7, No. 15, 22.04.2015, p. 8302-8312.

Research output: Contribution to journalArticle

Merkle, Valerie M. ; Martin, Daniel ; Hutchinson, Marcus ; Tran, Phat L. ; Behrens, Alana ; Hossainy, Samir ; Sheriff, Jawaad ; Bluestein, Danny ; Wu, Xiaoyi ; Slepian, Marvin J. / Hemocompatibility of poly(vinyl alcohol)-gelatin core-shell electrospun nanofibers : A scaffold for modulating platelet deposition and activation. In: ACS Applied Materials and Interfaces. 2015 ; Vol. 7, No. 15. pp. 8302-8312.
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