Hemorrhagic and necrotizing hepatitis associated with administration of a modified live canine adenovirus-2 vaccine in a maned wolf (Chrysocyon brachyurus)

Julie Swenson, Kathryn Orr, Gregory A Bradley

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A 15-yr-old, female, maned wolf (Chrysocyon brachyurus) was euthanized after presenting semicomatose with severe, uncontrolled frank hemorrhage from her rectum 6 days following a routine physical examination and vaccination. Histopathology indicated severe hemorrhagic and necrotizing hepatitis with intranuclear basophilic inclusion bodies in the liver that were thought to be consistent with adenoviral infection. Further classification by polymerase chain reaction, immunohistochemical staining, virus isolation, and electron microscopy confirmed the etiologic agent to be canine adenovirus-2. A representative sample of the vaccine that had been used was submitted and sequenced along with the virus isolated from the maned wolf. The sequencing of the etiologic agent that had been isolated from the maned wolf was determined to be the same as the strain of virus used in the production of the modified live vaccine that had been administered 6 days prior to death. From this information, the diagnosis of vaccine-induced adenoviral hepatitis was made. This is the first confirmed case of vaccine-induced canine adenoviral hepatitis in a maned wolf.

Original languageEnglish (US)
Pages (from-to)375-383
Number of pages9
JournalJournal of Zoo and Wildlife Medicine
Volume43
Issue number2
DOIs
StatePublished - Jun 2012

Fingerprint

Canine adenovirus A
hepatitis
Hepatitis
Canidae
vaccines
Vaccines
viruses
Viruses
nuclear inclusions
Canine Adenoviruses
live vaccines
Intranuclear Inclusion Bodies
rectum
histopathology
clinical examination
hemorrhage
electron microscopy
Rectum
Physical Examination
polymerase chain reaction

Keywords

  • Adenoviral hepatitis
  • Chrysocyon brachyurus
  • maned wolf
  • modified live virus vaccine
  • vaccine-induced

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

@article{76d22dc5674745cf927166e3b96ea2d9,
title = "Hemorrhagic and necrotizing hepatitis associated with administration of a modified live canine adenovirus-2 vaccine in a maned wolf (Chrysocyon brachyurus)",
abstract = "A 15-yr-old, female, maned wolf (Chrysocyon brachyurus) was euthanized after presenting semicomatose with severe, uncontrolled frank hemorrhage from her rectum 6 days following a routine physical examination and vaccination. Histopathology indicated severe hemorrhagic and necrotizing hepatitis with intranuclear basophilic inclusion bodies in the liver that were thought to be consistent with adenoviral infection. Further classification by polymerase chain reaction, immunohistochemical staining, virus isolation, and electron microscopy confirmed the etiologic agent to be canine adenovirus-2. A representative sample of the vaccine that had been used was submitted and sequenced along with the virus isolated from the maned wolf. The sequencing of the etiologic agent that had been isolated from the maned wolf was determined to be the same as the strain of virus used in the production of the modified live vaccine that had been administered 6 days prior to death. From this information, the diagnosis of vaccine-induced adenoviral hepatitis was made. This is the first confirmed case of vaccine-induced canine adenoviral hepatitis in a maned wolf.",
keywords = "Adenoviral hepatitis, Chrysocyon brachyurus, maned wolf, modified live virus vaccine, vaccine-induced",
author = "Julie Swenson and Kathryn Orr and Bradley, {Gregory A}",
year = "2012",
month = "6",
doi = "10.1638/2011-0192.1",
language = "English (US)",
volume = "43",
pages = "375--383",
journal = "Journal of Zoo and Wildlife Medicine",
issn = "1042-7260",
publisher = "American Association of Zoo Veterinarians",
number = "2",

}

TY - JOUR

T1 - Hemorrhagic and necrotizing hepatitis associated with administration of a modified live canine adenovirus-2 vaccine in a maned wolf (Chrysocyon brachyurus)

AU - Swenson, Julie

AU - Orr, Kathryn

AU - Bradley, Gregory A

PY - 2012/6

Y1 - 2012/6

N2 - A 15-yr-old, female, maned wolf (Chrysocyon brachyurus) was euthanized after presenting semicomatose with severe, uncontrolled frank hemorrhage from her rectum 6 days following a routine physical examination and vaccination. Histopathology indicated severe hemorrhagic and necrotizing hepatitis with intranuclear basophilic inclusion bodies in the liver that were thought to be consistent with adenoviral infection. Further classification by polymerase chain reaction, immunohistochemical staining, virus isolation, and electron microscopy confirmed the etiologic agent to be canine adenovirus-2. A representative sample of the vaccine that had been used was submitted and sequenced along with the virus isolated from the maned wolf. The sequencing of the etiologic agent that had been isolated from the maned wolf was determined to be the same as the strain of virus used in the production of the modified live vaccine that had been administered 6 days prior to death. From this information, the diagnosis of vaccine-induced adenoviral hepatitis was made. This is the first confirmed case of vaccine-induced canine adenoviral hepatitis in a maned wolf.

AB - A 15-yr-old, female, maned wolf (Chrysocyon brachyurus) was euthanized after presenting semicomatose with severe, uncontrolled frank hemorrhage from her rectum 6 days following a routine physical examination and vaccination. Histopathology indicated severe hemorrhagic and necrotizing hepatitis with intranuclear basophilic inclusion bodies in the liver that were thought to be consistent with adenoviral infection. Further classification by polymerase chain reaction, immunohistochemical staining, virus isolation, and electron microscopy confirmed the etiologic agent to be canine adenovirus-2. A representative sample of the vaccine that had been used was submitted and sequenced along with the virus isolated from the maned wolf. The sequencing of the etiologic agent that had been isolated from the maned wolf was determined to be the same as the strain of virus used in the production of the modified live vaccine that had been administered 6 days prior to death. From this information, the diagnosis of vaccine-induced adenoviral hepatitis was made. This is the first confirmed case of vaccine-induced canine adenoviral hepatitis in a maned wolf.

KW - Adenoviral hepatitis

KW - Chrysocyon brachyurus

KW - maned wolf

KW - modified live virus vaccine

KW - vaccine-induced

UR - http://www.scopus.com/inward/record.url?scp=84862891088&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862891088&partnerID=8YFLogxK

U2 - 10.1638/2011-0192.1

DO - 10.1638/2011-0192.1

M3 - Article

C2 - 22779244

AN - SCOPUS:84862891088

VL - 43

SP - 375

EP - 383

JO - Journal of Zoo and Wildlife Medicine

JF - Journal of Zoo and Wildlife Medicine

SN - 1042-7260

IS - 2

ER -