Hemostatic factors in rabbit limb lymph: Relationship to mechanisms regulating extravascular coagulation

Dzung T. Le, Peter Borgs, Thomas W. Toneff, Marlys H. Witte, Samuel I. Rapaport

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Mechanisms regulating extravascular coagulation in interstitial fluids of peripheral tissues are poorly understood, since measurements of hemostatic factors in these fluids are unavailable. Because lymph from a body region reflects the composition of its interstitial fluid, we measured hemostatic factors in limb lymph of rabbits both as activity and as antigen. Mean lymph- to-plasma activity ratios were the following: fibrinogen, 0.28; prothrombin, 0.26; factor X, 0.27; factor VII, 0.17; and factors V and VIII, 0.08. All lymph fibrinogen was clottable; fibrin degradation products were absent. Lymph von Willebrand factor antigen was <10% of plasma antigen and consisted primarily of lower molecular weight multimers. Mean lymph-to-plasma activity ratio for antithrombin was 0.38 and for tissue factor pathway inhibitor the ratio was 0.40. Low levels of antithrombin-factor Xa were measurable in lymph. The data are compatible with a basal factor VIIa-tissue factor- catalyzed extravascular activation of factor X that is prevented from progressing to generation of fibrin in limb interstitial fluid and lymph by low levels of factor VIII and factor V and by the inhibitory activity of antithrombin and tissue factor pathway inhibitor.

Original languageEnglish (US)
Pages (from-to)H769-H776
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume274
Issue number3 43-3
StatePublished - Mar 1 1998

Fingerprint

Lymph
Hemostatics
Extremities
Rabbits
Antithrombins
Extracellular Fluid
Factor X
Factor VIII
Fibrinogen
Fibrin Fibrinogen Degradation Products
Antigens
Factor VIIa
Body Regions
Factor VII
Factor Xa
Factor V
von Willebrand Factor
Thromboplastin
Prothrombin
Fibrin

Keywords

  • Antithrombin
  • Factor V
  • Factor VIII
  • Tissue factor pathway inhibitor
  • von Willebrand factor

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Hemostatic factors in rabbit limb lymph : Relationship to mechanisms regulating extravascular coagulation. / Le, Dzung T.; Borgs, Peter; Toneff, Thomas W.; Witte, Marlys H.; Rapaport, Samuel I.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 274, No. 3 43-3, 01.03.1998, p. H769-H776.

Research output: Contribution to journalArticle

@article{4e419dee3eb54b699472b58d3e83a339,
title = "Hemostatic factors in rabbit limb lymph: Relationship to mechanisms regulating extravascular coagulation",
abstract = "Mechanisms regulating extravascular coagulation in interstitial fluids of peripheral tissues are poorly understood, since measurements of hemostatic factors in these fluids are unavailable. Because lymph from a body region reflects the composition of its interstitial fluid, we measured hemostatic factors in limb lymph of rabbits both as activity and as antigen. Mean lymph- to-plasma activity ratios were the following: fibrinogen, 0.28; prothrombin, 0.26; factor X, 0.27; factor VII, 0.17; and factors V and VIII, 0.08. All lymph fibrinogen was clottable; fibrin degradation products were absent. Lymph von Willebrand factor antigen was <10{\%} of plasma antigen and consisted primarily of lower molecular weight multimers. Mean lymph-to-plasma activity ratio for antithrombin was 0.38 and for tissue factor pathway inhibitor the ratio was 0.40. Low levels of antithrombin-factor Xa were measurable in lymph. The data are compatible with a basal factor VIIa-tissue factor- catalyzed extravascular activation of factor X that is prevented from progressing to generation of fibrin in limb interstitial fluid and lymph by low levels of factor VIII and factor V and by the inhibitory activity of antithrombin and tissue factor pathway inhibitor.",
keywords = "Antithrombin, Factor V, Factor VIII, Tissue factor pathway inhibitor, von Willebrand factor",
author = "Le, {Dzung T.} and Peter Borgs and Toneff, {Thomas W.} and Witte, {Marlys H.} and Rapaport, {Samuel I.}",
year = "1998",
month = "3",
day = "1",
language = "English (US)",
volume = "274",
pages = "H769--H776",
journal = "American Journal of Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "3 43-3",

}

TY - JOUR

T1 - Hemostatic factors in rabbit limb lymph

T2 - Relationship to mechanisms regulating extravascular coagulation

AU - Le, Dzung T.

AU - Borgs, Peter

AU - Toneff, Thomas W.

AU - Witte, Marlys H.

AU - Rapaport, Samuel I.

PY - 1998/3/1

Y1 - 1998/3/1

N2 - Mechanisms regulating extravascular coagulation in interstitial fluids of peripheral tissues are poorly understood, since measurements of hemostatic factors in these fluids are unavailable. Because lymph from a body region reflects the composition of its interstitial fluid, we measured hemostatic factors in limb lymph of rabbits both as activity and as antigen. Mean lymph- to-plasma activity ratios were the following: fibrinogen, 0.28; prothrombin, 0.26; factor X, 0.27; factor VII, 0.17; and factors V and VIII, 0.08. All lymph fibrinogen was clottable; fibrin degradation products were absent. Lymph von Willebrand factor antigen was <10% of plasma antigen and consisted primarily of lower molecular weight multimers. Mean lymph-to-plasma activity ratio for antithrombin was 0.38 and for tissue factor pathway inhibitor the ratio was 0.40. Low levels of antithrombin-factor Xa were measurable in lymph. The data are compatible with a basal factor VIIa-tissue factor- catalyzed extravascular activation of factor X that is prevented from progressing to generation of fibrin in limb interstitial fluid and lymph by low levels of factor VIII and factor V and by the inhibitory activity of antithrombin and tissue factor pathway inhibitor.

AB - Mechanisms regulating extravascular coagulation in interstitial fluids of peripheral tissues are poorly understood, since measurements of hemostatic factors in these fluids are unavailable. Because lymph from a body region reflects the composition of its interstitial fluid, we measured hemostatic factors in limb lymph of rabbits both as activity and as antigen. Mean lymph- to-plasma activity ratios were the following: fibrinogen, 0.28; prothrombin, 0.26; factor X, 0.27; factor VII, 0.17; and factors V and VIII, 0.08. All lymph fibrinogen was clottable; fibrin degradation products were absent. Lymph von Willebrand factor antigen was <10% of plasma antigen and consisted primarily of lower molecular weight multimers. Mean lymph-to-plasma activity ratio for antithrombin was 0.38 and for tissue factor pathway inhibitor the ratio was 0.40. Low levels of antithrombin-factor Xa were measurable in lymph. The data are compatible with a basal factor VIIa-tissue factor- catalyzed extravascular activation of factor X that is prevented from progressing to generation of fibrin in limb interstitial fluid and lymph by low levels of factor VIII and factor V and by the inhibitory activity of antithrombin and tissue factor pathway inhibitor.

KW - Antithrombin

KW - Factor V

KW - Factor VIII

KW - Tissue factor pathway inhibitor

KW - von Willebrand factor

UR - http://www.scopus.com/inward/record.url?scp=33750871464&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750871464&partnerID=8YFLogxK

M3 - Article

C2 - 9530187

AN - SCOPUS:33750871464

VL - 274

SP - H769-H776

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6135

IS - 3 43-3

ER -