Herpes simplex virus type 1 ICP8: Helix-destabilizing properties

Paul E Boehmer, I. R. Lehman

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The major single-stranded DNA-binding protein, ICP8, of herpes simplex virus type 1 (HSV-1) is one of seven virus-encoded polypeptides required for HSV-1 DNA replication. To investigate the role of ICP8 in viral DNA replication, we have examined the interaction of ICP8 with partial DNA duplexes and found that it can displace oligonucleotides annealed to single-stranded M13 DNA. In addition, ICP8 can melt small fragments of fully duplex DNA. Unlike a DNA helicase, ICP8-promoted strand displacement is ATP and Mg2+ independent and exhibits no directionality. It requires saturating amounts of ICP8 and is both efficient and highly cooperative. These properties make ICP8 suitable for a role in DNA replication in which ICP8 destabilizes duplex DNA during origin unwinding and replication fork movement.

Original languageEnglish (US)
Pages (from-to)711-715
Number of pages5
JournalJournal of Virology
Volume67
Issue number2
StatePublished - Feb 1993
Externally publishedYes

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Human herpesvirus 1
Human Herpesvirus 1
DNA replication
DNA Replication
single-stranded DNA
DNA
DNA helicases
DNA Helicases
Replication Origin
DNA-binding proteins
Single-Stranded DNA
Viral DNA
DNA-Binding Proteins
oligonucleotides
Oligonucleotides
cooperatives
polypeptides
Adenosine Triphosphate
Viruses
viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Herpes simplex virus type 1 ICP8 : Helix-destabilizing properties. / Boehmer, Paul E; Lehman, I. R.

In: Journal of Virology, Vol. 67, No. 2, 02.1993, p. 711-715.

Research output: Contribution to journalArticle

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