Heterogeneity in the Effect of Albumin and Other Resuscitation Fluids on Intracellular Oxygen Free Radical Production

Cuthbert O. Simpkins, Donnie Little, Andrew Brenner, January A. Hill, John A. Griswold, Peter M Rhee, Carl J. Hauser, Henry J. Schiller, Cathy L. White-Owen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: We hypothesized that by studying a suspension of a single cell type in various resuscitation fluids, we could compare their effects on parameters associated with cell death. Methods: Jurkat cells were suspended in resuscitation fluids. Using flow cytometry, light scatter, phosphatidylserine translocation, propidium iodide uptake, and intracellular H2O 2 production were measured. Results: Buffer (pH, 7.4) and albumin added to Ringer's lactate inhibited the adverse changes, including intracellular oxygen free radical production. Oxygen free radical production was variable within the cell population and inhibited by albumin but not other colloids or crystalloids. This correlated well with the ability of albumin to enhance metabolic activity. A flavoprotein inhibitor blocked H2O 2 production, suggesting that mitochondria are the source of the H2O2 and the variability. Conclusion: Oxygen free radical inhibition by albumin could explain both its beneficial and its harmful effects.

Original languageEnglish (US)
Pages (from-to)548-559
Number of pages12
JournalJournal of Trauma
Volume56
Issue number3
StatePublished - Mar 2004
Externally publishedYes

Fingerprint

Intracellular Fluid
Resuscitation
Free Radicals
Albumins
Reactive Oxygen Species
Flavoproteins
Jurkat Cells
Propidium
Phosphatidylserines
Colloids
Suspensions
Flow Cytometry
Buffers
Mitochondria
Cell Death
Light
Population

Keywords

  • Albumin
  • Jurkat cells
  • Lymphocytes
  • Oxygen free radicals
  • Resuscitation fluids

ASJC Scopus subject areas

  • Surgery

Cite this

Simpkins, C. O., Little, D., Brenner, A., Hill, J. A., Griswold, J. A., Rhee, P. M., ... White-Owen, C. L. (2004). Heterogeneity in the Effect of Albumin and Other Resuscitation Fluids on Intracellular Oxygen Free Radical Production. Journal of Trauma, 56(3), 548-559.

Heterogeneity in the Effect of Albumin and Other Resuscitation Fluids on Intracellular Oxygen Free Radical Production. / Simpkins, Cuthbert O.; Little, Donnie; Brenner, Andrew; Hill, January A.; Griswold, John A.; Rhee, Peter M; Hauser, Carl J.; Schiller, Henry J.; White-Owen, Cathy L.

In: Journal of Trauma, Vol. 56, No. 3, 03.2004, p. 548-559.

Research output: Contribution to journalArticle

Simpkins, CO, Little, D, Brenner, A, Hill, JA, Griswold, JA, Rhee, PM, Hauser, CJ, Schiller, HJ & White-Owen, CL 2004, 'Heterogeneity in the Effect of Albumin and Other Resuscitation Fluids on Intracellular Oxygen Free Radical Production', Journal of Trauma, vol. 56, no. 3, pp. 548-559.
Simpkins, Cuthbert O. ; Little, Donnie ; Brenner, Andrew ; Hill, January A. ; Griswold, John A. ; Rhee, Peter M ; Hauser, Carl J. ; Schiller, Henry J. ; White-Owen, Cathy L. / Heterogeneity in the Effect of Albumin and Other Resuscitation Fluids on Intracellular Oxygen Free Radical Production. In: Journal of Trauma. 2004 ; Vol. 56, No. 3. pp. 548-559.
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AU - Griswold, John A.

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