Heterogeneity, polyploidy, aneusomy, and 9p deletion in human glioblastoma multiforme

Se Hyuck Park, Tatsuhiro Maeda, Gayatry Mohapatra, Frederic M. Waldman, Richard L. Davis, Burt G.F. Feuerstein

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The short arm of chromosome 9 is frequently deleted in malignant gliomas. We used locus-specific probes for interferon-A (IFNA) and D9S3 in combination with a chromosome 9 centromeric probe to detect genetic aberrations on a cell-by-cell basis in touch preparations of 30 glioblastomas by fluorescence in situ hybridization. Seven (23%) of 30 tumors had deletions in >70% of cells; the IFNA locus was deleted in all seven, but the D9S3 locus was deleted in only five of the seven. The latter data confirm that a tumor suppressor gene on 9p relevant to glioblastoma multiforme lies between D9S3 and IFNA. Eleven tumors had deletions in 20-40% of cells, more than three standard deviations above the level in control tissues. The remaining tumors had deletions in < 20% of cells. The seven tumors with the lowest percentage of deleted cells each had more than one geentically abnormal population of cells. In total, 10 cases were of this type (i.e., aneusomic for chromosome 9). Three of these 10 tumors had hybridization patterns consistent with polyploidy.

Original languageEnglish (US)
Pages (from-to)127-135
Number of pages9
JournalCancer Genetics and Cytogenetics
Volume83
Issue number2
DOIs
StatePublished - 1995
Externally publishedYes

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Polyploidy
Glioblastoma
Chromosomes, Human, Pair 9
Interferons
Neoplasms
Touch
Tumor Suppressor Genes
Fluorescence In Situ Hybridization
Glioma
Population

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Heterogeneity, polyploidy, aneusomy, and 9p deletion in human glioblastoma multiforme. / Park, Se Hyuck; Maeda, Tatsuhiro; Mohapatra, Gayatry; Waldman, Frederic M.; Davis, Richard L.; Feuerstein, Burt G.F.

In: Cancer Genetics and Cytogenetics, Vol. 83, No. 2, 1995, p. 127-135.

Research output: Contribution to journalArticle

Park, Se Hyuck ; Maeda, Tatsuhiro ; Mohapatra, Gayatry ; Waldman, Frederic M. ; Davis, Richard L. ; Feuerstein, Burt G.F. / Heterogeneity, polyploidy, aneusomy, and 9p deletion in human glioblastoma multiforme. In: Cancer Genetics and Cytogenetics. 1995 ; Vol. 83, No. 2. pp. 127-135.
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AU - Davis, Richard L.

AU - Feuerstein, Burt G.F.

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