Heterorhabditis sonorensis n. sp. (Nematoda

Heterorhabditidae), a natural pathogen of the seasonal cicada Diceroprocta ornea (Walker) (Homoptera: Cicadidae) in the Sonoran desert

S Patricia Stock, Benjamin Rivera-Orduño, Yolanda Flores-Lara

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A new Heterorhabditis species was isolated from nymphal stages of the seasonal cicada Diceroprocta ornea (Walker) in an asparagus field in the state of Sonora, Mexico. Concomitantly, another isolate of the same nematode species was also collected from an oak woodland habitat in the Chiricahua mountain range in southeastern Arizona. Morphological and molecular studies together with cross-hybridization tests indicate these two isolates are conspecific and represent a new undescribed Heterorhabditis sp. This new species is distinguished from other species in this genus by a combination of several qualitative and quantitative morphological traits. Key diagnostic features include: presence of a pronounced post-anal swelling in the hermaphrodite; male with nine pairs of bursal rays, with pairs 4 and 7 bent outwards and one pair of papillae placed on the cloacal opening, value of D% (average: 79); infective juveniles with a well developed cuticular tooth, long tail (average: 105 μm) and values of D% (average: 90) and E% (average: 99). In addition to these diagnostic characters, cross-hybridization tests between the new species with H. bacteriophora and H. mexicana yielded no fertile progeny. Comparison of ITS rDNA sequences with other available sequences of described species depicted the two isolates as a new species. Phylogenetic analysis of these sequence data placed H. sonorensis n. sp. as a member of the indica-group.

Original languageEnglish (US)
Pages (from-to)175-184
Number of pages10
JournalJournal of Invertebrate Pathology
Volume100
Issue number3
DOIs
StatePublished - Mar 2009

Fingerprint

Diceroprocta
Heterorhabditidae
Heterorhabditis
Cicadidae
Sonoran Desert
Homoptera
pathogen
desert
Nematoda
new species
pathogens
hybridization
nematode larvae
hermaphrodite
woodlands
teeth
Quercus
tail
Mexico
testing

Keywords

  • Arizona
  • Heterorhabditis sonorensis n. sp. - cicada - asparagus - oak woodlands
  • Sonora

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Cite this

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title = "Heterorhabditis sonorensis n. sp. (Nematoda: Heterorhabditidae), a natural pathogen of the seasonal cicada Diceroprocta ornea (Walker) (Homoptera: Cicadidae) in the Sonoran desert",
abstract = "A new Heterorhabditis species was isolated from nymphal stages of the seasonal cicada Diceroprocta ornea (Walker) in an asparagus field in the state of Sonora, Mexico. Concomitantly, another isolate of the same nematode species was also collected from an oak woodland habitat in the Chiricahua mountain range in southeastern Arizona. Morphological and molecular studies together with cross-hybridization tests indicate these two isolates are conspecific and represent a new undescribed Heterorhabditis sp. This new species is distinguished from other species in this genus by a combination of several qualitative and quantitative morphological traits. Key diagnostic features include: presence of a pronounced post-anal swelling in the hermaphrodite; male with nine pairs of bursal rays, with pairs 4 and 7 bent outwards and one pair of papillae placed on the cloacal opening, value of D{\%} (average: 79); infective juveniles with a well developed cuticular tooth, long tail (average: 105 μm) and values of D{\%} (average: 90) and E{\%} (average: 99). In addition to these diagnostic characters, cross-hybridization tests between the new species with H. bacteriophora and H. mexicana yielded no fertile progeny. Comparison of ITS rDNA sequences with other available sequences of described species depicted the two isolates as a new species. Phylogenetic analysis of these sequence data placed H. sonorensis n. sp. as a member of the indica-group.",
keywords = "Arizona, Heterorhabditis sonorensis n. sp. - cicada - asparagus - oak woodlands, Sonora",
author = "Stock, {S Patricia} and Benjamin Rivera-Ordu{\~n}o and Yolanda Flores-Lara",
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volume = "100",
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T1 - Heterorhabditis sonorensis n. sp. (Nematoda

T2 - Heterorhabditidae), a natural pathogen of the seasonal cicada Diceroprocta ornea (Walker) (Homoptera: Cicadidae) in the Sonoran desert

AU - Stock, S Patricia

AU - Rivera-Orduño, Benjamin

AU - Flores-Lara, Yolanda

PY - 2009/3

Y1 - 2009/3

N2 - A new Heterorhabditis species was isolated from nymphal stages of the seasonal cicada Diceroprocta ornea (Walker) in an asparagus field in the state of Sonora, Mexico. Concomitantly, another isolate of the same nematode species was also collected from an oak woodland habitat in the Chiricahua mountain range in southeastern Arizona. Morphological and molecular studies together with cross-hybridization tests indicate these two isolates are conspecific and represent a new undescribed Heterorhabditis sp. This new species is distinguished from other species in this genus by a combination of several qualitative and quantitative morphological traits. Key diagnostic features include: presence of a pronounced post-anal swelling in the hermaphrodite; male with nine pairs of bursal rays, with pairs 4 and 7 bent outwards and one pair of papillae placed on the cloacal opening, value of D% (average: 79); infective juveniles with a well developed cuticular tooth, long tail (average: 105 μm) and values of D% (average: 90) and E% (average: 99). In addition to these diagnostic characters, cross-hybridization tests between the new species with H. bacteriophora and H. mexicana yielded no fertile progeny. Comparison of ITS rDNA sequences with other available sequences of described species depicted the two isolates as a new species. Phylogenetic analysis of these sequence data placed H. sonorensis n. sp. as a member of the indica-group.

AB - A new Heterorhabditis species was isolated from nymphal stages of the seasonal cicada Diceroprocta ornea (Walker) in an asparagus field in the state of Sonora, Mexico. Concomitantly, another isolate of the same nematode species was also collected from an oak woodland habitat in the Chiricahua mountain range in southeastern Arizona. Morphological and molecular studies together with cross-hybridization tests indicate these two isolates are conspecific and represent a new undescribed Heterorhabditis sp. This new species is distinguished from other species in this genus by a combination of several qualitative and quantitative morphological traits. Key diagnostic features include: presence of a pronounced post-anal swelling in the hermaphrodite; male with nine pairs of bursal rays, with pairs 4 and 7 bent outwards and one pair of papillae placed on the cloacal opening, value of D% (average: 79); infective juveniles with a well developed cuticular tooth, long tail (average: 105 μm) and values of D% (average: 90) and E% (average: 99). In addition to these diagnostic characters, cross-hybridization tests between the new species with H. bacteriophora and H. mexicana yielded no fertile progeny. Comparison of ITS rDNA sequences with other available sequences of described species depicted the two isolates as a new species. Phylogenetic analysis of these sequence data placed H. sonorensis n. sp. as a member of the indica-group.

KW - Arizona

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