Hierarchical extensibility in the PEVK domain of skeletal-muscle titin

A. Nagy, L. Grama, T. Huber, P. Bianco, K. Trombitás, Hendrikus "Henk" Granzier, M. S Z Kellermayer

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Titin is the main determinant of passive muscle force. Physiological extension of titin derives largely from its PEVK (Pro-Glu-Val-Lys) domain, which has a different length in different muscle types. Here we characterized the elasticity of the full-length, human soleus PEVK domain by mechanically manipulating its contiguous, recombinant subdomain segments: an N-terminal (PEVKI), a middle (PEVKII), and a C-terminal (PEVKIII) one third. Measurement of the apparent persistence lengths revealed a hierarchical arrangement according to local flexibility: the N-terminal PEVKI is the most rigid and the C-terminal PEVKIII is the most flexible segment within the domain, Immunoelectron microscopy supported the hierarchical extensibility within the PEVK domain. The effective persistence lengths decreased as a function of ionic strength, as predicted by the Odijk-Skolnick-Fixman model of polyelectrolyte chains. The ionic strength dependence of persistence length was similar in all segments, indicating that the residual differences in the elasticity of the segments derive from nonelectrostatic mechanisms.

Original languageEnglish (US)
Pages (from-to)329-336
Number of pages8
JournalBiophysical Journal
Volume89
Issue number1
DOIs
StatePublished - Jul 2005
Externally publishedYes

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Connectin
Elasticity
Osmolar Concentration
Skeletal Muscle
Muscles
Immunoelectron Microscopy
Polyelectrolytes

ASJC Scopus subject areas

  • Biophysics

Cite this

Nagy, A., Grama, L., Huber, T., Bianco, P., Trombitás, K., Granzier, H. H., & Kellermayer, M. S. Z. (2005). Hierarchical extensibility in the PEVK domain of skeletal-muscle titin. Biophysical Journal, 89(1), 329-336. https://doi.org/10.1529/biophysj.104.057737

Hierarchical extensibility in the PEVK domain of skeletal-muscle titin. / Nagy, A.; Grama, L.; Huber, T.; Bianco, P.; Trombitás, K.; Granzier, Hendrikus "Henk"; Kellermayer, M. S Z.

In: Biophysical Journal, Vol. 89, No. 1, 07.2005, p. 329-336.

Research output: Contribution to journalArticle

Nagy, A, Grama, L, Huber, T, Bianco, P, Trombitás, K, Granzier, HH & Kellermayer, MSZ 2005, 'Hierarchical extensibility in the PEVK domain of skeletal-muscle titin', Biophysical Journal, vol. 89, no. 1, pp. 329-336. https://doi.org/10.1529/biophysj.104.057737
Nagy, A. ; Grama, L. ; Huber, T. ; Bianco, P. ; Trombitás, K. ; Granzier, Hendrikus "Henk" ; Kellermayer, M. S Z. / Hierarchical extensibility in the PEVK domain of skeletal-muscle titin. In: Biophysical Journal. 2005 ; Vol. 89, No. 1. pp. 329-336.
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