High efficiency gene delivery into laryngeal muscle with bidirectional electroporation

Koichiro Saito, Mohamed Lehar, Zhao Bo Li, Natasha Braga, Alan D. King, Robin A. Samlan, Paul W. Flint

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Objective: The impact of polarity change on the efficiency of in vivo electroporative (EP) gene transfection was assessed in rat laryngeal muscle. Study design and setting: High (HV) and low field voltage (LV) were combined with polarity changes to determine transfection in 5 different conditions: 1) without EP (EP[-]), 2) HV+LV (HL), 3) HV+LV followed by HV+LV with no change in polarity (HLHL unidirectional), 4) HV+LV followed by HV+LV with opposite polarity (HLHL bidirectional), 5) HV+LV followed by LV with opposite polarity (HLL bidirectional). Results: HLL bidirectional sequence showed the best result with less interindividual variability and extended expression period. With the exception of repeated high voltage sequences, EP parameters were not likely to induce cell injury or inflammation. Conclusion: HLL bidirectional electroporative gene delivery produces high transfection rates with limited tissue trauma. Significance: Bidirectional EP provides a safe and highly efficient method for therapeutic gene delivery into skeletal muscle.

Original languageEnglish (US)
Pages (from-to)209-214
Number of pages6
JournalOtolaryngology - Head and Neck Surgery
Volume135
Issue number2
DOIs
StatePublished - Aug 6 2006
Externally publishedYes

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

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