High-frequency reactivation of X-linked genes in chinese hamster × human hybrid cells

Nathan Ellis, Elisabeth Keitges, Stanley M. Gartler, Mariano Rocchi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Three genes on the human inactive X chromosome retained in the Chinese hamster x human hybrid cell line X8/6T2 have been reactivated using the demethylating agent, 5-azacytidine (5-aza-CR). Pulse-labeling and histochemical methods permitted detection and measurement of reactivation rates of the hypoxanthine phosphoribosyltransferase (Hpt) and glucose-6-phosphate dehydrogenase (G6pd) genes within 48 h of treatment. About 50% of the cells became active for these genes, which represents a reactivation rate some 30-fold greater than previously reported in similar systems. The phosphoglycerate kinase (Pgk) gene was not reactivated as frequently as the Hpt or G6pd genes. Segregation analysis of progeny of treated cells showed that enzyme-positive and enzyme-negative cells were produced in proportions supporting the notion that 5-aza-CR causes demethylation by replicative loss and that demethylation leads to reactivation.

Original languageEnglish (US)
Pages (from-to)191-204
Number of pages14
JournalSomatic Cell and Molecular Genetics
Volume13
Issue number3
DOIs
StatePublished - May 1987
Externally publishedYes

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X-Linked Genes
Hybrid Cells
Cricetulus
Hypoxanthine Phosphoribosyltransferase
Glucosephosphate Dehydrogenase
Genes
Chromosomes, Human, X
Phosphoglycerate Kinase
Azacitidine
Enzymes
Cell Line

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

High-frequency reactivation of X-linked genes in chinese hamster × human hybrid cells. / Ellis, Nathan; Keitges, Elisabeth; Gartler, Stanley M.; Rocchi, Mariano.

In: Somatic Cell and Molecular Genetics, Vol. 13, No. 3, 05.1987, p. 191-204.

Research output: Contribution to journalArticle

Ellis, Nathan ; Keitges, Elisabeth ; Gartler, Stanley M. ; Rocchi, Mariano. / High-frequency reactivation of X-linked genes in chinese hamster × human hybrid cells. In: Somatic Cell and Molecular Genetics. 1987 ; Vol. 13, No. 3. pp. 191-204.
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