High-performance liquid chromatographic analysis of biogenic amines in biological materials as o-phthalaldehyde derivatives

Thomas P. Davis, Charles W. Gehrke, Charles W. Gehrke, Thomas D. Cunningham, Kenneth C. Kuo, Klaus O. Gerhardt, Harold D. Johnson, Charles H. Williams

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

A remarkably sensitive, simple and selective reversed-phase high-performance liquid chromatographic (HPLC) method has been developed, allowing, for the first time, the direct measurement of histamine, norepinephrine, octopamine, normetanephrine, dopamine, serotonin and tyramine in a single sample of plasma (2 ml), tissue (0.2 g), or urine. The biogenic amines were modified by pre-column derivatization with o-phthalaldehyde which stabilizes the molecules, aids in extraction, and improves HPLC detection at the nanogram level. To minimize losses during the sampling procedure a careful collection procedure was designed.

Original languageEnglish (US)
Pages (from-to)293-310
Number of pages18
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume162
Issue number3
DOIs
StatePublished - Mar 1 1979

ASJC Scopus subject areas

  • Chemistry(all)

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