High-performance liquid chromatographic analysis of biogenic amines in biological materials as o-phthalaldehyde derivatives

Thomas P Davis, Charles W. Gehrke, Charles W. Gehrke, Thomas D. Cunningham, Kenneth C. Kuo, Klaus O. Gerhardt, Harold D. Johnson, Charles H. Williams

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

A remarkably sensitive, simple and selective reversed-phase high-performance liquid chromatographic (HPLC) method has been developed, allowing, for the first time, the direct measurement of histamine, norepinephrine, octopamine, normetanephrine, dopamine, serotonin and tyramine in a single sample of plasma (2 ml), tissue (0.2 g), or urine. The biogenic amines were modified by pre-column derivatization with o-phthalaldehyde which stabilizes the molecules, aids in extraction, and improves HPLC detection at the nanogram level. To minimize losses during the sampling procedure a careful collection procedure was designed.

Original languageEnglish (US)
Pages (from-to)293-310
Number of pages18
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume162
Issue number3
DOIs
StatePublished - Mar 1 1979
Externally publishedYes

Fingerprint

o-Phthalaldehyde
Biogenic Amines
High performance liquid chromatography
Biological materials
High Pressure Liquid Chromatography
Normetanephrine
Octopamine
Derivatives
Tyramine
Liquids
Histamine
Dopamine
Serotonin
Norepinephrine
Tissue
Sampling
Plasmas
Molecules
Urine

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

High-performance liquid chromatographic analysis of biogenic amines in biological materials as o-phthalaldehyde derivatives. / Davis, Thomas P; Gehrke, Charles W.; Gehrke, Charles W.; Cunningham, Thomas D.; Kuo, Kenneth C.; Gerhardt, Klaus O.; Johnson, Harold D.; Williams, Charles H.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 162, No. 3, 01.03.1979, p. 293-310.

Research output: Contribution to journalArticle

Davis, Thomas P ; Gehrke, Charles W. ; Gehrke, Charles W. ; Cunningham, Thomas D. ; Kuo, Kenneth C. ; Gerhardt, Klaus O. ; Johnson, Harold D. ; Williams, Charles H. / High-performance liquid chromatographic analysis of biogenic amines in biological materials as o-phthalaldehyde derivatives. In: Journal of Chromatography B: Biomedical Sciences and Applications. 1979 ; Vol. 162, No. 3. pp. 293-310.
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