High performance liquid chromatographic analysis of MS23 piperidine analog MSP001 in rat plasma

Ting Wang, Desuo Wang

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

MSP001 is a newly synthesized piperidine analog of the lead antihypertensive compound MS23 that dually targets cAMP-specific type 4 phosphodiesterase and L-type calcium channels. We validated an analytical protocol for MSP001 in rat plasma using high performance liquid chromatographic method. A C18 column and a phosphate/acetonitrile buffer were used to perform the chromatographic separation. UV detection was carried out at 307 nm, a wavelength at which an absorption peak was detected for this group of compounds. The calibration curve for MSP001 was linear in the range from 25 to 10,000 ng/ml. The limit of quantification (LOQ) was 25 ng/ml. The results demonstrate that this method has high linearity (R = 0.99995), compound specificity, and acceptable precision/accuracy. The protocol is suitable for in vivo pharmacokinetic studies on the compound.

Original languageEnglish (US)
Pages (from-to)607-612
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume42
Issue number5
DOIs
StatePublished - Nov 16 2006
Externally publishedYes

Keywords

  • Antihypertensive
  • Dual action
  • MS23
  • MSP001
  • Validation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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