High performance liquid chromatographic analysis of MS23 piperidine analog MSP001 in rat plasma

Ting Wang, Desuo Wang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

MSP001 is a newly synthesized piperidine analog of the lead antihypertensive compound MS23 that dually targets cAMP-specific type 4 phosphodiesterase and L-type calcium channels. We validated an analytical protocol for MSP001 in rat plasma using high performance liquid chromatographic method. A C18 column and a phosphate/acetonitrile buffer were used to perform the chromatographic separation. UV detection was carried out at 307 nm, a wavelength at which an absorption peak was detected for this group of compounds. The calibration curve for MSP001 was linear in the range from 25 to 10,000 ng/ml. The limit of quantification (LOQ) was 25 ng/ml. The results demonstrate that this method has high linearity (R = 0.99995), compound specificity, and acceptable precision/accuracy. The protocol is suitable for in vivo pharmacokinetic studies on the compound.

Original languageEnglish (US)
Pages (from-to)607-612
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume42
Issue number5
DOIs
StatePublished - Nov 16 2006
Externally publishedYes

Fingerprint

High performance liquid chromatography
Rats
High Pressure Liquid Chromatography
Plasmas
Type 4 Cyclic Nucleotide Phosphodiesterase
Lead compounds
L-Type Calcium Channels
Pharmacokinetics
Antihypertensive Agents
Calibration
Buffers
Phosphates
Wavelength
Liquids
MSP001
piperidine

Keywords

  • Antihypertensive
  • Dual action
  • MS23
  • MSP001
  • Validation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science

Cite this

High performance liquid chromatographic analysis of MS23 piperidine analog MSP001 in rat plasma. / Wang, Ting; Wang, Desuo.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 42, No. 5, 16.11.2006, p. 607-612.

Research output: Contribution to journalArticle

@article{ab8f08f2b53f47d0b1f5524de701311b,
title = "High performance liquid chromatographic analysis of MS23 piperidine analog MSP001 in rat plasma",
abstract = "MSP001 is a newly synthesized piperidine analog of the lead antihypertensive compound MS23 that dually targets cAMP-specific type 4 phosphodiesterase and L-type calcium channels. We validated an analytical protocol for MSP001 in rat plasma using high performance liquid chromatographic method. A C18 column and a phosphate/acetonitrile buffer were used to perform the chromatographic separation. UV detection was carried out at 307 nm, a wavelength at which an absorption peak was detected for this group of compounds. The calibration curve for MSP001 was linear in the range from 25 to 10,000 ng/ml. The limit of quantification (LOQ) was 25 ng/ml. The results demonstrate that this method has high linearity (R = 0.99995), compound specificity, and acceptable precision/accuracy. The protocol is suitable for in vivo pharmacokinetic studies on the compound.",
keywords = "Antihypertensive, Dual action, MS23, MSP001, Validation",
author = "Ting Wang and Desuo Wang",
year = "2006",
month = "11",
day = "16",
doi = "10.1016/j.jpba.2006.05.010",
language = "English (US)",
volume = "42",
pages = "607--612",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
issn = "0731-7085",
publisher = "Elsevier",
number = "5",

}

TY - JOUR

T1 - High performance liquid chromatographic analysis of MS23 piperidine analog MSP001 in rat plasma

AU - Wang, Ting

AU - Wang, Desuo

PY - 2006/11/16

Y1 - 2006/11/16

N2 - MSP001 is a newly synthesized piperidine analog of the lead antihypertensive compound MS23 that dually targets cAMP-specific type 4 phosphodiesterase and L-type calcium channels. We validated an analytical protocol for MSP001 in rat plasma using high performance liquid chromatographic method. A C18 column and a phosphate/acetonitrile buffer were used to perform the chromatographic separation. UV detection was carried out at 307 nm, a wavelength at which an absorption peak was detected for this group of compounds. The calibration curve for MSP001 was linear in the range from 25 to 10,000 ng/ml. The limit of quantification (LOQ) was 25 ng/ml. The results demonstrate that this method has high linearity (R = 0.99995), compound specificity, and acceptable precision/accuracy. The protocol is suitable for in vivo pharmacokinetic studies on the compound.

AB - MSP001 is a newly synthesized piperidine analog of the lead antihypertensive compound MS23 that dually targets cAMP-specific type 4 phosphodiesterase and L-type calcium channels. We validated an analytical protocol for MSP001 in rat plasma using high performance liquid chromatographic method. A C18 column and a phosphate/acetonitrile buffer were used to perform the chromatographic separation. UV detection was carried out at 307 nm, a wavelength at which an absorption peak was detected for this group of compounds. The calibration curve for MSP001 was linear in the range from 25 to 10,000 ng/ml. The limit of quantification (LOQ) was 25 ng/ml. The results demonstrate that this method has high linearity (R = 0.99995), compound specificity, and acceptable precision/accuracy. The protocol is suitable for in vivo pharmacokinetic studies on the compound.

KW - Antihypertensive

KW - Dual action

KW - MS23

KW - MSP001

KW - Validation

UR - http://www.scopus.com/inward/record.url?scp=33750329605&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750329605&partnerID=8YFLogxK

U2 - 10.1016/j.jpba.2006.05.010

DO - 10.1016/j.jpba.2006.05.010

M3 - Article

VL - 42

SP - 607

EP - 612

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

IS - 5

ER -