High-performance liquid chromatography of peptides at reduced temperatures: Separation of isomers

Michal Lebl, Sunan Fang, Victor J Hruby

Research output: Contribution to journalArticle

13 Scopus citations


Cholecystokinin analogues containing N-methyl amino acids were studied by reversed-phase high-performance liquid chromatography at reduced temperatures. A reduction in temperature to - 17°C led to lower efficiency, but at the same time separations of cis and trans isomers (and some impurities) were achieved. The velocity constants for cis-trans equilibria were calculated.

Original languageEnglish (US)
Pages (from-to)145-148
Number of pages4
JournalJournal of Chromatography A
Issue number1
Publication statusPublished - Nov 8 1991


ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

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