High-sensitivity confocal imaging with bilateral scanning and CCD detectors

G. J. Brakenhoff, Koen Visscher

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The high quantum efficiency and high red sensitivity of a charge coupled device (CCD) make it a very suitable detector for confocal fluorescence imaging, especially for applications in biology, where short wavelength illumination may cause undesirable radiation damage. In addition, its high dynamic range matches the high contrast imaging inherent in confocal microscopy, and enables one to record small intensity differencesif this dynamic range is well exploited. Applications shown include imaging with bleaching rate and non-linear fluorescence excitation as parameters. The bilateral scan technique permits effective confocal imaging using this type of device. CCDs can be used for confocal imaging both in high speed (up to real-time) applications as well as in the integration mode, where the high sensitivity application areas for this confocal technique are present.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Int Soc for Optical Engineering
Pages521-531
Number of pages11
Editionpt 2
ISBN (Print)081940814X
StatePublished - Dec 1 1992
Externally publishedYes
EventBiomedical Image Processing and Three-Dimensional Microscopy. Part 2 (of 2) - San Jose, CA, USA
Duration: Feb 10 1992Feb 13 1992

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Numberpt 2
Volume1660
ISSN (Print)0277-786X

Other

OtherBiomedical Image Processing and Three-Dimensional Microscopy. Part 2 (of 2)
CitySan Jose, CA, USA
Period2/10/922/13/92

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'High-sensitivity confocal imaging with bilateral scanning and CCD detectors'. Together they form a unique fingerprint.

Cite this