High-sensitivity confocal imaging with bilateral scanning and CCD detectors

G. J. Brakenhoff, Koen Visscher

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The high quantum efficiency and high red sensitivity of a charge coupled device (CCD) make it a very suitable detector for confocal fluorescence imaging, especially for applications in biology, where short wavelength illumination may cause undesirable radiation damage. In addition, its high dynamic range matches the high contrast imaging inherent in confocal microscopy, and enables one to record small intensity differencesif this dynamic range is well exploited. Applications shown include imaging with bleaching rate and non-linear fluorescence excitation as parameters. The bilateral scan technique permits effective confocal imaging using this type of device. CCDs can be used for confocal imaging both in high speed (up to real-time) applications as well as in the integration mode, where the high sensitivity application areas for this confocal technique are present.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Int Soc for Optical Engineering
Pages521-531
Number of pages11
Volume1660
Editionpt 2
ISBN (Print)081940814X
StatePublished - 1992
Externally publishedYes
EventBiomedical Image Processing and Three-Dimensional Microscopy. Part 2 (of 2) - San Jose, CA, USA
Duration: Feb 10 1992Feb 13 1992

Other

OtherBiomedical Image Processing and Three-Dimensional Microscopy. Part 2 (of 2)
CitySan Jose, CA, USA
Period2/10/922/13/92

Fingerprint

Charge coupled devices
charge coupled devices
Detectors
Scanning
Imaging techniques
scanning
sensitivity
detectors
dynamic range
fluorescence
Fluorescence
bleaching
biology
radiation damage
Confocal microscopy
Radiation damage
quantum efficiency
Bleaching
Quantum efficiency
illumination

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Brakenhoff, G. J., & Visscher, K. (1992). High-sensitivity confocal imaging with bilateral scanning and CCD detectors. In Proceedings of SPIE - The International Society for Optical Engineering (pt 2 ed., Vol. 1660, pp. 521-531). Publ by Int Soc for Optical Engineering.

High-sensitivity confocal imaging with bilateral scanning and CCD detectors. / Brakenhoff, G. J.; Visscher, Koen.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1660 pt 2. ed. Publ by Int Soc for Optical Engineering, 1992. p. 521-531.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Brakenhoff, GJ & Visscher, K 1992, High-sensitivity confocal imaging with bilateral scanning and CCD detectors. in Proceedings of SPIE - The International Society for Optical Engineering. pt 2 edn, vol. 1660, Publ by Int Soc for Optical Engineering, pp. 521-531, Biomedical Image Processing and Three-Dimensional Microscopy. Part 2 (of 2), San Jose, CA, USA, 2/10/92.
Brakenhoff GJ, Visscher K. High-sensitivity confocal imaging with bilateral scanning and CCD detectors. In Proceedings of SPIE - The International Society for Optical Engineering. pt 2 ed. Vol. 1660. Publ by Int Soc for Optical Engineering. 1992. p. 521-531
Brakenhoff, G. J. ; Visscher, Koen. / High-sensitivity confocal imaging with bilateral scanning and CCD detectors. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1660 pt 2. ed. Publ by Int Soc for Optical Engineering, 1992. pp. 521-531
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