High-speed capillary zone electrophoresis with online photolytic optical injection

Suminda Hapuarachchi, Sean P. Premeau, Craig A Aspinwall

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We report an online, optical injection interface for capillary zone electrophoresis (CZE) based upon photophysical activation of a caged, fluorogenic label covalently attached to the target analyte. This injection interface allows online analysis of biomolecular systems with high temporal resolution and high sensitivity. Samples are injected onto the separation capillary by photolysis of a caged-fluorescein label using the 351-364 nm irradiation of an Ar+ laser. Following injection, the sample is separated and detected via laser-induced fluorescence detection at 488 nm. Detection limits for online analysis of arginine, glutamic acid, and aspartic acid were less than 1 nM with separation times less than 5 s and separation efficiencies exceeding 1 000 000 plates/m. Rapid injection of proteins was demonstrated with migration times less than 500 ms and 0.5 nM detection limits. Online monitoring was performed with response times less than 20 s, suggesting the feasibility of this approach for online, in vivo analysis for a range of biologically relevant analytes.

Original languageEnglish (US)
Pages (from-to)3674-3680
Number of pages7
JournalAnalytical Chemistry
Volume78
Issue number11
DOIs
StatePublished - Jun 1 2006

Fingerprint

Electrophoresis
Labels
Lasers
Photolysis
Fluorescein
Aspartic Acid
Arginine
Glutamic Acid
Fluorescence
Chemical activation
Irradiation
Monitoring
Proteins

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

High-speed capillary zone electrophoresis with online photolytic optical injection. / Hapuarachchi, Suminda; Premeau, Sean P.; Aspinwall, Craig A.

In: Analytical Chemistry, Vol. 78, No. 11, 01.06.2006, p. 3674-3680.

Research output: Contribution to journalArticle

Hapuarachchi, Suminda ; Premeau, Sean P. ; Aspinwall, Craig A. / High-speed capillary zone electrophoresis with online photolytic optical injection. In: Analytical Chemistry. 2006 ; Vol. 78, No. 11. pp. 3674-3680.
@article{9f783451806b42acb81a4baf54e16fd5,
title = "High-speed capillary zone electrophoresis with online photolytic optical injection",
abstract = "We report an online, optical injection interface for capillary zone electrophoresis (CZE) based upon photophysical activation of a caged, fluorogenic label covalently attached to the target analyte. This injection interface allows online analysis of biomolecular systems with high temporal resolution and high sensitivity. Samples are injected onto the separation capillary by photolysis of a caged-fluorescein label using the 351-364 nm irradiation of an Ar+ laser. Following injection, the sample is separated and detected via laser-induced fluorescence detection at 488 nm. Detection limits for online analysis of arginine, glutamic acid, and aspartic acid were less than 1 nM with separation times less than 5 s and separation efficiencies exceeding 1 000 000 plates/m. Rapid injection of proteins was demonstrated with migration times less than 500 ms and 0.5 nM detection limits. Online monitoring was performed with response times less than 20 s, suggesting the feasibility of this approach for online, in vivo analysis for a range of biologically relevant analytes.",
author = "Suminda Hapuarachchi and Premeau, {Sean P.} and Aspinwall, {Craig A}",
year = "2006",
month = "6",
day = "1",
doi = "10.1021/ac051645q",
language = "English (US)",
volume = "78",
pages = "3674--3680",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "11",

}

TY - JOUR

T1 - High-speed capillary zone electrophoresis with online photolytic optical injection

AU - Hapuarachchi, Suminda

AU - Premeau, Sean P.

AU - Aspinwall, Craig A

PY - 2006/6/1

Y1 - 2006/6/1

N2 - We report an online, optical injection interface for capillary zone electrophoresis (CZE) based upon photophysical activation of a caged, fluorogenic label covalently attached to the target analyte. This injection interface allows online analysis of biomolecular systems with high temporal resolution and high sensitivity. Samples are injected onto the separation capillary by photolysis of a caged-fluorescein label using the 351-364 nm irradiation of an Ar+ laser. Following injection, the sample is separated and detected via laser-induced fluorescence detection at 488 nm. Detection limits for online analysis of arginine, glutamic acid, and aspartic acid were less than 1 nM with separation times less than 5 s and separation efficiencies exceeding 1 000 000 plates/m. Rapid injection of proteins was demonstrated with migration times less than 500 ms and 0.5 nM detection limits. Online monitoring was performed with response times less than 20 s, suggesting the feasibility of this approach for online, in vivo analysis for a range of biologically relevant analytes.

AB - We report an online, optical injection interface for capillary zone electrophoresis (CZE) based upon photophysical activation of a caged, fluorogenic label covalently attached to the target analyte. This injection interface allows online analysis of biomolecular systems with high temporal resolution and high sensitivity. Samples are injected onto the separation capillary by photolysis of a caged-fluorescein label using the 351-364 nm irradiation of an Ar+ laser. Following injection, the sample is separated and detected via laser-induced fluorescence detection at 488 nm. Detection limits for online analysis of arginine, glutamic acid, and aspartic acid were less than 1 nM with separation times less than 5 s and separation efficiencies exceeding 1 000 000 plates/m. Rapid injection of proteins was demonstrated with migration times less than 500 ms and 0.5 nM detection limits. Online monitoring was performed with response times less than 20 s, suggesting the feasibility of this approach for online, in vivo analysis for a range of biologically relevant analytes.

UR - http://www.scopus.com/inward/record.url?scp=33744947074&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744947074&partnerID=8YFLogxK

U2 - 10.1021/ac051645q

DO - 10.1021/ac051645q

M3 - Article

C2 - 16737223

AN - SCOPUS:33744947074

VL - 78

SP - 3674

EP - 3680

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 11

ER -