High-throughput, high-sensitivity analysis of gene expression in arabidopsis

Richard Martin Kris, Stephen Felder, Michael Deyholos, Georgina M. Lambert, James Hinton, Ihab Botros, Ralph Martel, Bruce Seligmann, David W. Galbraith

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

High-throughput gene expression analysis of genes expressed during salt stress was performed using a novel multiplexed quantitative nuclease protection assay that involves customized DNA microarrays printed within the individual wells of 96-well plates. The levels of expression of the transcripts from 16 different genes were quantified within crude homogenates prepared from Arabidopsis (Arabidopsis thaliana) plants also grown in a 96-well plate format. Examples are provided of the high degree of reproducibility of quantitative dose-response data and of the sensitivity of detection of changes in gene expression within limiting amounts of tissue. The lack of requirement for RNA purification renders the assay particularly suited for high-throughput gene expression analysis and for the discovery of novel chemical compounds that specifically modulate the expression of endogenous target genes.

Original languageEnglish (US)
Pages (from-to)1256-1266
Number of pages11
JournalPlant physiology
Volume144
Issue number3
DOIs
StatePublished - Jul 1 2007

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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    Kris, R. M., Felder, S., Deyholos, M., Lambert, G. M., Hinton, J., Botros, I., Martel, R., Seligmann, B., & Galbraith, D. W. (2007). High-throughput, high-sensitivity analysis of gene expression in arabidopsis. Plant physiology, 144(3), 1256-1266. https://doi.org/10.1104/pp.107.098681