Higher expression of the heterogeneous nuclear ribonucleoprotein K in melanoma

Fushi Wen, Alex Shen, Reneé Shanas, Achyut Bhattacharyya, Fangru Lian, Galen Hostetter, Jiaqi Shi

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26 Scopus citations

Abstract

Background: The heterogeneous nuclear ribonucleoprotein (hnRNP) K is an essential RNA and DNA binding protein involved in gene expression and signal transduction. The role of hnRNP K in cancer is relatively understudied. However, several cellular functions strongly indicate that hnRNP K is involved in tumorigenesis. Oncogenes c-Src, c-myc, and eIF4E are regulated by hnRNP K. We have shown an increased cytoplasmic hnRNP K in pancreatic cancer. In the present study, we investigated the altered expression of hnRNP K protein and its correlation with p-ERK in melanoma using human melanoma cell lines and tissue microarray. Materials and Methods: The protein levels of hnRNP K and p-ERK in 8 human melanoma cell lines and a melanoma progression tissue microarray containing 80 melanoma, 23 dysplastic nevi, and 14 benign nevi specimens were analyzed using Western blot and immunohistochemistry analysis. hnRNP K was knocked down by siRNA, and its effect on melanoma cells was assessed. Results: We showed a higher hnRNP K protein level in both melanoma cell lines and melanoma tissue specimens, which correlated with a higher c-myc expression. An increase in the cytoplasmic hnRNP K and eIF4E protein levels in melanoma cells is also seen. p-ERK level was also higher in dysplastic nevi and melanoma tissues, but did not correlate with hnRNP K protein level. We then demonstrated that knocking down of hnRNP K by siRNA inhibited melanoma cell growth and colony formation, as well as c-myc expression. Conclusions: hnRNP K expression correlated with melanoma and may play a role in melanoma tumorigenesis.

Original languageEnglish (US)
Pages (from-to)2619-2627
Number of pages9
JournalAnnals of Surgical Oncology
Volume17
Issue number10
DOIs
StatePublished - Oct 1 2010

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ASJC Scopus subject areas

  • Surgery
  • Oncology

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