Highly simplified method for gas-liquid chromatographic quantitation of bile acids and sterols in human stool

Ashok Kumar Batta, Gerald Salen, Keshav R. Rapole, Manju Batta, Priti Batta, David Alberts, David Earnest

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

A simple method for the gas-liquid chromatographic quantitation of human fecal bile acids and sterols is described where bile acids are subjected to n-butyl ester derivatization, without prior isolation from the stool, followed by trimethylsilylation of the sterols and bile acids. Under these conditions, bile acid derivatives are well resolved from each other and from the trimethylsilyl ether derivatives of fecal sterols and no overlap occurs. The method was shown to be highly reproducible and recoveries were similar to those obtained with other methods used for fecal bile acid analysis. Application of the method for bile acid and sterol analysis in human stool is described.

Original languageEnglish (US)
Pages (from-to)1148-1154
Number of pages7
JournalJournal of Lipid Research
Volume40
Issue number6
StatePublished - Jun 1 1999

Keywords

  • Bile acid n-butyl ester-trimethyl-silyl ethers
  • Bile acids
  • Capillary gas- liquid chromatography
  • Fatty acids
  • Fecal sterols
  • Human fecal bile acids

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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  • Cite this

    Batta, A. K., Salen, G., Rapole, K. R., Batta, M., Batta, P., Alberts, D., & Earnest, D. (1999). Highly simplified method for gas-liquid chromatographic quantitation of bile acids and sterols in human stool. Journal of Lipid Research, 40(6), 1148-1154.