Histone deacetylase 1 (HDAC1) participates in the down-regulation of corticotropin releasing hormone gene (crh) expression

Lydia Miller, Chad D. Foradori, Avin S. Lalmansingh, Dharmendra Sharma, Robert J Handa, Rosalie M. Uht

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The paraventricular nucleus of the hypothalamus (PVH) plays a central role in regulating the hypothalamic-pituitary-adrenal (HPA) axis. Medial parvocellular neurons of the PVH (mpPVH) integrate sensory and humoral inputs to maintain homeostasis. Humoral inputs include glucocorticoids secreted by the adrenals, which down-regulate HPA activation. A primary glucocorticoid target is the population of mpPVH neurons that synthesize and secrete corticotropin-releasing factors, the most potent of which is corticotropin-releasing hormone (CRH). Although CRH gene (crh) expression is known to be down-regulated by glucocorticoids, the mechanisms by which this process occurs are still poorly understood. To begin this study we postulated that glucocorticoid repression of crh involves HDAC recruitment to the region of the crh proximal promoter. To evaluate this hypothesis, we treated hypothalamic cells that express CRH with the HDAC inhibitor trichostatin A (TSA). As predicted, treatment with TSA led to increased CRH mRNA levels and crh promoter activity. Although co-treatment with Dex (10-7M) reduced the TSA effect on mRNA levels, it failed to reduce promoter activity; however co-transfection of HDAC1 but not 3 restored Dex inhibition. A distinction between HDAC1 and 3 was also apparent with respect to crh promoter occupancy. Dex led to increased HDAC1 but not HDAC3 occupancy. In vivo studies revealed that CRH-immunoreactive (-ir) neurons contained HDAC1- and HDAC3-ir. Collectively, these data point to a role for HDAC1 in the physiologic regulation of crh.

Original languageEnglish (US)
Pages (from-to)312-320
Number of pages9
JournalPhysiology and Behavior
Volume104
Issue number2
DOIs
StatePublished - Aug 3 2011

Fingerprint

Histone Deacetylase 1
Corticotropin-Releasing Hormone
Down-Regulation
Gene Expression
trichostatin A
Glucocorticoids
Genes
Neurons
Paraventricular Hypothalamic Nucleus
Hypothalamus
Messenger RNA
Histone Deacetylase Inhibitors
Health Services Needs and Demand

Keywords

  • Corticotropin releasing factor
  • Gene expression
  • Histone deacetylase 1

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Experimental and Cognitive Psychology

Cite this

Histone deacetylase 1 (HDAC1) participates in the down-regulation of corticotropin releasing hormone gene (crh) expression. / Miller, Lydia; Foradori, Chad D.; Lalmansingh, Avin S.; Sharma, Dharmendra; Handa, Robert J; Uht, Rosalie M.

In: Physiology and Behavior, Vol. 104, No. 2, 03.08.2011, p. 312-320.

Research output: Contribution to journalArticle

Miller, Lydia ; Foradori, Chad D. ; Lalmansingh, Avin S. ; Sharma, Dharmendra ; Handa, Robert J ; Uht, Rosalie M. / Histone deacetylase 1 (HDAC1) participates in the down-regulation of corticotropin releasing hormone gene (crh) expression. In: Physiology and Behavior. 2011 ; Vol. 104, No. 2. pp. 312-320.
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