Histopathological characterization and in situ detection of Callinectes sapidus reovirus

Kathy F.J. Tang, Gretchen A. Messick, Carlos R. Pantoja, Rita M. Redman, Donald V. Lightner

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

A reovirus (tentatively designated as Callinectes sapidus reovirus, CsRV) was found in the blue crabs C. sapidus collected in Chesapeake Bay in 2005. Histological examination of hepatopancreas and gill from infected crabs revealed eosinophilic to basophilic, cytoplasmic, inclusions in hemocytes and in cells of connective tissue. A cDNA library was constructed from total RNA extracted from hemolymph of infected crabs. One clone (designated as CsRV-28) with a 532-bp insert was 75% identical in nucleotide sequence (and 95% similar in translated amino acid sequence) to the quanylytransferase gene of the Scylla serrata reovirus (SsRV). The insert of CsRV-28 was labeled with digoxigenin-11-dUTP and hybridized to sections of hepatopancreas and gill of infected C. sapidus, this probe reacted to hemocytes and cells in the connective tissue. No reaction was seen in any of the tissues prepared from uninfected crabs. Thus, this in situ hybridization procedure can be used to diagnose CsRV.

Original languageEnglish (US)
Pages (from-to)226-228
Number of pages3
JournalJournal of Invertebrate Pathology
Volume108
Issue number3
DOIs
StatePublished - Nov 2011

Keywords

  • Callinectes sapidus reovirus
  • In situ hybridization
  • Viral guanylytransferase gene

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

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