HIV-1 replication and gene expression occur at higher levels in neonatal blood naive and memory T-lymphocytes compared with adult blood cells

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Abstract

Our previous study has shown that HIV-1 replicated at higher levels in neonatal (cord) blood monocytes/macrophages and T-lymphocytes compared with adult blood cells. However, it is not known whether this differential HIV-1 replication also occurs in naive and/or memory T-lymphocytes. We, therefore, compared HIV-1 replication in CD3+ and CD4+ naive (CD45RA+) and memory (CD45RO+) T-lymphocytes isolated from five cord and adult blood donors. We found that HIV-1 replicated at higher levels in both CD3+ and CD4+ CD45RA+ and CD45RO+ T-lymphocytes isolated from cord blood compared with adult blood. In addition, there was no difference in the cell surface expression of CD4, CXCR4 and CCR5 on cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells. Furthermore, we found that there was an increase in HIV-1 gene expression in cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells by using a single-cycle replication competent HIV-1-NL4-3-Env-R+ luciferase amphotropic virus, which measures HIV-1 transcriptional activity independent of CD4 and CXCR4 or CCR5 expression. In summary, HIV-1 replicated at higher levels in cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells and this differential replication is influenced at the level of HIV-1 gene expression.

Original languageEnglish (US)
Pages (from-to)39-46
Number of pages8
JournalVirology
Volume413
Issue number1
DOIs
StatePublished - Apr 25 2011

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HIV-1
Blood Cells
T-Lymphocytes
Fetal Blood
Gene Expression
Blood Donors
Luciferases
Monocytes
Macrophages
Viruses

Keywords

  • CD4
  • Cord and adult blood naive and memory T-lymphocytes
  • CXCR4 and CCR5 expression on naive and memory T-lymphocytes
  • HIV-1 gene expression
  • HIV-1 replication in naive and memory T-lymphocytes

ASJC Scopus subject areas

  • Virology

Cite this

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title = "HIV-1 replication and gene expression occur at higher levels in neonatal blood naive and memory T-lymphocytes compared with adult blood cells",
abstract = "Our previous study has shown that HIV-1 replicated at higher levels in neonatal (cord) blood monocytes/macrophages and T-lymphocytes compared with adult blood cells. However, it is not known whether this differential HIV-1 replication also occurs in naive and/or memory T-lymphocytes. We, therefore, compared HIV-1 replication in CD3+ and CD4+ naive (CD45RA+) and memory (CD45RO+) T-lymphocytes isolated from five cord and adult blood donors. We found that HIV-1 replicated at higher levels in both CD3+ and CD4+ CD45RA+ and CD45RO+ T-lymphocytes isolated from cord blood compared with adult blood. In addition, there was no difference in the cell surface expression of CD4, CXCR4 and CCR5 on cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells. Furthermore, we found that there was an increase in HIV-1 gene expression in cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells by using a single-cycle replication competent HIV-1-NL4-3-Env-R+ luciferase amphotropic virus, which measures HIV-1 transcriptional activity independent of CD4 and CXCR4 or CCR5 expression. In summary, HIV-1 replicated at higher levels in cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells and this differential replication is influenced at the level of HIV-1 gene expression.",
keywords = "CD4, Cord and adult blood naive and memory T-lymphocytes, CXCR4 and CCR5 expression on naive and memory T-lymphocytes, HIV-1 gene expression, HIV-1 replication in naive and memory T-lymphocytes",
author = "Ahmad, {Nafees -} and Roshni Mehta and Harris, {David T.}",
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doi = "10.1016/j.virol.2011.01.033",
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T1 - HIV-1 replication and gene expression occur at higher levels in neonatal blood naive and memory T-lymphocytes compared with adult blood cells

AU - Ahmad, Nafees -

AU - Mehta, Roshni

AU - Harris, David T.

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N2 - Our previous study has shown that HIV-1 replicated at higher levels in neonatal (cord) blood monocytes/macrophages and T-lymphocytes compared with adult blood cells. However, it is not known whether this differential HIV-1 replication also occurs in naive and/or memory T-lymphocytes. We, therefore, compared HIV-1 replication in CD3+ and CD4+ naive (CD45RA+) and memory (CD45RO+) T-lymphocytes isolated from five cord and adult blood donors. We found that HIV-1 replicated at higher levels in both CD3+ and CD4+ CD45RA+ and CD45RO+ T-lymphocytes isolated from cord blood compared with adult blood. In addition, there was no difference in the cell surface expression of CD4, CXCR4 and CCR5 on cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells. Furthermore, we found that there was an increase in HIV-1 gene expression in cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells by using a single-cycle replication competent HIV-1-NL4-3-Env-R+ luciferase amphotropic virus, which measures HIV-1 transcriptional activity independent of CD4 and CXCR4 or CCR5 expression. In summary, HIV-1 replicated at higher levels in cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells and this differential replication is influenced at the level of HIV-1 gene expression.

AB - Our previous study has shown that HIV-1 replicated at higher levels in neonatal (cord) blood monocytes/macrophages and T-lymphocytes compared with adult blood cells. However, it is not known whether this differential HIV-1 replication also occurs in naive and/or memory T-lymphocytes. We, therefore, compared HIV-1 replication in CD3+ and CD4+ naive (CD45RA+) and memory (CD45RO+) T-lymphocytes isolated from five cord and adult blood donors. We found that HIV-1 replicated at higher levels in both CD3+ and CD4+ CD45RA+ and CD45RO+ T-lymphocytes isolated from cord blood compared with adult blood. In addition, there was no difference in the cell surface expression of CD4, CXCR4 and CCR5 on cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells. Furthermore, we found that there was an increase in HIV-1 gene expression in cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells by using a single-cycle replication competent HIV-1-NL4-3-Env-R+ luciferase amphotropic virus, which measures HIV-1 transcriptional activity independent of CD4 and CXCR4 or CCR5 expression. In summary, HIV-1 replicated at higher levels in cord blood CD45RA+ and CD45RO+ T-lymphocytes compared with adult blood cells and this differential replication is influenced at the level of HIV-1 gene expression.

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