Holographic optical coherence imaging of rat osteogenic sarcoma tumor spheroids

Ping Yu, Mirela Mustata, Leilei Peng, John J. Turek, Michael R. Melloch, Paul M W French, David D. Nolte

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Holographic optical coherence imaging is a full-frame variant of coherence-domain imaging. An optoelectronic semiconductor holographic film functions as a coherence filter placed before a conventional digital video camera that passes coherent (structure-bearing) light to the camera during holographic readout while preferentially rejecting scattered light. The data are acquired as a succession of en face images at increasing depth inside the sample in a fly-through acquisition. The samples of living tissue were rat osteogenic sarcoma multicellular tumor spheroids that were grown from a single osteoblast cell line in a bioreaotor. Tumor spheroids are nearly spherical and have radial symmetry, presenting a simple geometry for analysis. The tumors investigated ranged in diameter from several hundred micrometers to over 1 mm. Holographic features from the tumors were observed in reflection to depths of 500-600 μm with a total tissue path length of approximately 14 mean free paths. The volumetric data from the tumor spheroids reveal heterogeneous structure, presumably caused by necrosis and microcalcifications characteristic of some human avascular tumors.

Original languageEnglish (US)
Pages (from-to)4862-4873
Number of pages12
JournalApplied Optics
Volume43
Issue number25
DOIs
StatePublished - Sep 1 2004
Externally publishedYes

Fingerprint

spheroids
rats
Rats
Tumors
tumors
cancer
Imaging techniques
Bearings (structural)
cameras
Tissue
osteoblasts
necrosis
Osteoblasts
Digital cameras
Video cameras
cultured cells
mean free path
Optoelectronic devices
readout
micrometers

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Yu, P., Mustata, M., Peng, L., Turek, J. J., Melloch, M. R., French, P. M. W., & Nolte, D. D. (2004). Holographic optical coherence imaging of rat osteogenic sarcoma tumor spheroids. Applied Optics, 43(25), 4862-4873. https://doi.org/10.1364/AO.43.004862

Holographic optical coherence imaging of rat osteogenic sarcoma tumor spheroids. / Yu, Ping; Mustata, Mirela; Peng, Leilei; Turek, John J.; Melloch, Michael R.; French, Paul M W; Nolte, David D.

In: Applied Optics, Vol. 43, No. 25, 01.09.2004, p. 4862-4873.

Research output: Contribution to journalArticle

Yu, P, Mustata, M, Peng, L, Turek, JJ, Melloch, MR, French, PMW & Nolte, DD 2004, 'Holographic optical coherence imaging of rat osteogenic sarcoma tumor spheroids', Applied Optics, vol. 43, no. 25, pp. 4862-4873. https://doi.org/10.1364/AO.43.004862
Yu, Ping ; Mustata, Mirela ; Peng, Leilei ; Turek, John J. ; Melloch, Michael R. ; French, Paul M W ; Nolte, David D. / Holographic optical coherence imaging of rat osteogenic sarcoma tumor spheroids. In: Applied Optics. 2004 ; Vol. 43, No. 25. pp. 4862-4873.
@article{0738ab6f1a194751aadfea22e4d81cbb,
title = "Holographic optical coherence imaging of rat osteogenic sarcoma tumor spheroids",
abstract = "Holographic optical coherence imaging is a full-frame variant of coherence-domain imaging. An optoelectronic semiconductor holographic film functions as a coherence filter placed before a conventional digital video camera that passes coherent (structure-bearing) light to the camera during holographic readout while preferentially rejecting scattered light. The data are acquired as a succession of en face images at increasing depth inside the sample in a fly-through acquisition. The samples of living tissue were rat osteogenic sarcoma multicellular tumor spheroids that were grown from a single osteoblast cell line in a bioreaotor. Tumor spheroids are nearly spherical and have radial symmetry, presenting a simple geometry for analysis. The tumors investigated ranged in diameter from several hundred micrometers to over 1 mm. Holographic features from the tumors were observed in reflection to depths of 500-600 μm with a total tissue path length of approximately 14 mean free paths. The volumetric data from the tumor spheroids reveal heterogeneous structure, presumably caused by necrosis and microcalcifications characteristic of some human avascular tumors.",
author = "Ping Yu and Mirela Mustata and Leilei Peng and Turek, {John J.} and Melloch, {Michael R.} and French, {Paul M W} and Nolte, {David D.}",
year = "2004",
month = "9",
day = "1",
doi = "10.1364/AO.43.004862",
language = "English (US)",
volume = "43",
pages = "4862--4873",
journal = "Applied Optics",
issn = "1559-128X",
publisher = "The Optical Society",
number = "25",

}

TY - JOUR

T1 - Holographic optical coherence imaging of rat osteogenic sarcoma tumor spheroids

AU - Yu, Ping

AU - Mustata, Mirela

AU - Peng, Leilei

AU - Turek, John J.

AU - Melloch, Michael R.

AU - French, Paul M W

AU - Nolte, David D.

PY - 2004/9/1

Y1 - 2004/9/1

N2 - Holographic optical coherence imaging is a full-frame variant of coherence-domain imaging. An optoelectronic semiconductor holographic film functions as a coherence filter placed before a conventional digital video camera that passes coherent (structure-bearing) light to the camera during holographic readout while preferentially rejecting scattered light. The data are acquired as a succession of en face images at increasing depth inside the sample in a fly-through acquisition. The samples of living tissue were rat osteogenic sarcoma multicellular tumor spheroids that were grown from a single osteoblast cell line in a bioreaotor. Tumor spheroids are nearly spherical and have radial symmetry, presenting a simple geometry for analysis. The tumors investigated ranged in diameter from several hundred micrometers to over 1 mm. Holographic features from the tumors were observed in reflection to depths of 500-600 μm with a total tissue path length of approximately 14 mean free paths. The volumetric data from the tumor spheroids reveal heterogeneous structure, presumably caused by necrosis and microcalcifications characteristic of some human avascular tumors.

AB - Holographic optical coherence imaging is a full-frame variant of coherence-domain imaging. An optoelectronic semiconductor holographic film functions as a coherence filter placed before a conventional digital video camera that passes coherent (structure-bearing) light to the camera during holographic readout while preferentially rejecting scattered light. The data are acquired as a succession of en face images at increasing depth inside the sample in a fly-through acquisition. The samples of living tissue were rat osteogenic sarcoma multicellular tumor spheroids that were grown from a single osteoblast cell line in a bioreaotor. Tumor spheroids are nearly spherical and have radial symmetry, presenting a simple geometry for analysis. The tumors investigated ranged in diameter from several hundred micrometers to over 1 mm. Holographic features from the tumors were observed in reflection to depths of 500-600 μm with a total tissue path length of approximately 14 mean free paths. The volumetric data from the tumor spheroids reveal heterogeneous structure, presumably caused by necrosis and microcalcifications characteristic of some human avascular tumors.

UR - http://www.scopus.com/inward/record.url?scp=4544225833&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4544225833&partnerID=8YFLogxK

U2 - 10.1364/AO.43.004862

DO - 10.1364/AO.43.004862

M3 - Article

C2 - 15449473

AN - SCOPUS:4544225833

VL - 43

SP - 4862

EP - 4873

JO - Applied Optics

JF - Applied Optics

SN - 1559-128X

IS - 25

ER -