Homologues of insecticidal toxin complex genes within a genomic island in the marine bacterium Vibrio parahaemolyticus

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Abstract

Three insecticidal toxin complex (tc)-like genes were identified in Vibrio parahaemolyticus 13-028/A3, which can cause acute hepatopancreatic necrosis disease in penaeid shrimp. The three genes are a tcdA-like gene (7710 bp), predicted to code for a 284-kDa protein; a tcdB-like gene (4272 bp), predicted to code for a 158-kDa protein; and a tccC3-like gene (2916 bp), predicted to encode a 107-kDa protein. All three predicted proteins contain conserved domains that are characteristic of their respective Tc proteins. By RT-PCR, all three tc-like genes were found to be expressed in this bacterium. Through genome walking and the use of PCR to join contigs surrounding these three genes, a genomic island (87 712 bp, named tc-GIvp) was found on chromosome II localized next to the tRNA Gly. The GC content of this island, which is not found in other Vibrio species, is 40%. The tc-GIvp is characterized to have 60 ORFs encoding regulatory or virulence factors. These include a type 6 secretion protein VgrG, EAL domain-containing proteins, fimbriae subunits and assembly proteins, invasin-like proteins, peptidoglycan-binding proteins, and Tc proteins. The tc-GIvp also contains 21 transposase genes, suggesting that it was acquired through horizontal transfer from other organisms. Three insecticidal toxin complex-like genes were identified in a marine bacterium Vibrio parahaemolyticus.

Original languageEnglish (US)
Pages (from-to)34-42
Number of pages9
JournalFEMS Microbiology Letters
Volume361
Issue number1
DOIs
Publication statusPublished - Dec 1 2014

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Keywords

  • Vibrio parahaemolyticus
  • Genomic island
  • Insecticidal toxin complex genes

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

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