Homozygous deletions of the CDKN2 (MTS1/p16ink4) gene in cell lines established from children with acute lymphoblastic leukemia

M. Zhou, L. Gu, C. D. James, J. He, Andrew M Yeager, S. D. Smith, H. W. Findley

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Homozygous deletions of the CDKN2 (MTS1/p16ink4) gene have been found at high frequency in cell lines derived from a variety of adult solid tumors. In order to investigate the status of the CDKN2 gene in cell lines established from childhood acute lymphoblastic leukemia (ALL), we surveyed 25 lines representing the major pediatric ALL phenotypes for the presence of this gene by Southern blot analysis. Homozygous deletions of all or part of the CDKN2 gene were detected in 21 (84%) cell lines, including 11 of 14 (79%) early-pre-B-ALL, four of five (80%) pre-B-ALL, and six of six T-ALL lines. CDKN2 mRNA was detected by Northern blotting in each of the four lines containing an intact CDKN2 gene. These data suggest an important role for CDKN2 deletion in the cause and/or progression of pediatric ALL.

Original languageEnglish (US)
Pages (from-to)1159-1161
Number of pages3
JournalLeukemia
Volume9
Issue number7
StatePublished - Jul 1995
Externally publishedYes

Fingerprint

p16 Genes
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Cell Line
Pediatrics
Southern Blotting
Northern Blotting
Phenotype
Messenger RNA
Genes
Neoplasms

Keywords

  • CDKN2 deletion
  • Pediatric leukemia
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Cancer Research
  • Hematology

Cite this

Zhou, M., Gu, L., James, C. D., He, J., Yeager, A. M., Smith, S. D., & Findley, H. W. (1995). Homozygous deletions of the CDKN2 (MTS1/p16ink4) gene in cell lines established from children with acute lymphoblastic leukemia. Leukemia, 9(7), 1159-1161.

Homozygous deletions of the CDKN2 (MTS1/p16ink4) gene in cell lines established from children with acute lymphoblastic leukemia. / Zhou, M.; Gu, L.; James, C. D.; He, J.; Yeager, Andrew M; Smith, S. D.; Findley, H. W.

In: Leukemia, Vol. 9, No. 7, 07.1995, p. 1159-1161.

Research output: Contribution to journalArticle

Zhou, M, Gu, L, James, CD, He, J, Yeager, AM, Smith, SD & Findley, HW 1995, 'Homozygous deletions of the CDKN2 (MTS1/p16ink4) gene in cell lines established from children with acute lymphoblastic leukemia', Leukemia, vol. 9, no. 7, pp. 1159-1161.
Zhou, M. ; Gu, L. ; James, C. D. ; He, J. ; Yeager, Andrew M ; Smith, S. D. ; Findley, H. W. / Homozygous deletions of the CDKN2 (MTS1/p16ink4) gene in cell lines established from children with acute lymphoblastic leukemia. In: Leukemia. 1995 ; Vol. 9, No. 7. pp. 1159-1161.
@article{40fa5f9ed0cc4948994a459dd9218062,
title = "Homozygous deletions of the CDKN2 (MTS1/p16ink4) gene in cell lines established from children with acute lymphoblastic leukemia",
abstract = "Homozygous deletions of the CDKN2 (MTS1/p16ink4) gene have been found at high frequency in cell lines derived from a variety of adult solid tumors. In order to investigate the status of the CDKN2 gene in cell lines established from childhood acute lymphoblastic leukemia (ALL), we surveyed 25 lines representing the major pediatric ALL phenotypes for the presence of this gene by Southern blot analysis. Homozygous deletions of all or part of the CDKN2 gene were detected in 21 (84{\%}) cell lines, including 11 of 14 (79{\%}) early-pre-B-ALL, four of five (80{\%}) pre-B-ALL, and six of six T-ALL lines. CDKN2 mRNA was detected by Northern blotting in each of the four lines containing an intact CDKN2 gene. These data suggest an important role for CDKN2 deletion in the cause and/or progression of pediatric ALL.",
keywords = "CDKN2 deletion, Pediatric leukemia, Tumor suppressor gene",
author = "M. Zhou and L. Gu and James, {C. D.} and J. He and Yeager, {Andrew M} and Smith, {S. D.} and Findley, {H. W.}",
year = "1995",
month = "7",
language = "English (US)",
volume = "9",
pages = "1159--1161",
journal = "Leukemia",
issn = "0887-6924",
publisher = "Nature Publishing Group",
number = "7",

}

TY - JOUR

T1 - Homozygous deletions of the CDKN2 (MTS1/p16ink4) gene in cell lines established from children with acute lymphoblastic leukemia

AU - Zhou, M.

AU - Gu, L.

AU - James, C. D.

AU - He, J.

AU - Yeager, Andrew M

AU - Smith, S. D.

AU - Findley, H. W.

PY - 1995/7

Y1 - 1995/7

N2 - Homozygous deletions of the CDKN2 (MTS1/p16ink4) gene have been found at high frequency in cell lines derived from a variety of adult solid tumors. In order to investigate the status of the CDKN2 gene in cell lines established from childhood acute lymphoblastic leukemia (ALL), we surveyed 25 lines representing the major pediatric ALL phenotypes for the presence of this gene by Southern blot analysis. Homozygous deletions of all or part of the CDKN2 gene were detected in 21 (84%) cell lines, including 11 of 14 (79%) early-pre-B-ALL, four of five (80%) pre-B-ALL, and six of six T-ALL lines. CDKN2 mRNA was detected by Northern blotting in each of the four lines containing an intact CDKN2 gene. These data suggest an important role for CDKN2 deletion in the cause and/or progression of pediatric ALL.

AB - Homozygous deletions of the CDKN2 (MTS1/p16ink4) gene have been found at high frequency in cell lines derived from a variety of adult solid tumors. In order to investigate the status of the CDKN2 gene in cell lines established from childhood acute lymphoblastic leukemia (ALL), we surveyed 25 lines representing the major pediatric ALL phenotypes for the presence of this gene by Southern blot analysis. Homozygous deletions of all or part of the CDKN2 gene were detected in 21 (84%) cell lines, including 11 of 14 (79%) early-pre-B-ALL, four of five (80%) pre-B-ALL, and six of six T-ALL lines. CDKN2 mRNA was detected by Northern blotting in each of the four lines containing an intact CDKN2 gene. These data suggest an important role for CDKN2 deletion in the cause and/or progression of pediatric ALL.

KW - CDKN2 deletion

KW - Pediatric leukemia

KW - Tumor suppressor gene

UR - http://www.scopus.com/inward/record.url?scp=0029134873&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029134873&partnerID=8YFLogxK

M3 - Article

C2 - 7630190

AN - SCOPUS:0029134873

VL - 9

SP - 1159

EP - 1161

JO - Leukemia

JF - Leukemia

SN - 0887-6924

IS - 7

ER -