Honeybees (Apis mellifera) learn to discriminate the smell of organic compounds from their respective deuterated isotopomers

Wulfila Gronenberg, Ajay Raikhelkar, Eric Abshire, Jennifer Stevens, Eric Epstein, Karin Loyola, Michael Rauscher, Stephen Buchmann

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The understanding of physiological and molecular processes underlying the sense of smell has made considerable progress during the past three decades, revealing the cascade of molecular steps that lead to the activation of olfactory receptor (OR) neurons. However, the mode of primary interaction of odorant molecules with the OR proteins within the sensory cells is still enigmatic. Two different concepts try to explain these interactions: the 'odotope hypothesis' suggests that OR proteins recognize structural aspects of the odorant molecule, whereas the 'vibration hypothesis' proposes that intra-molecular vibrations are the basis for the recognition of the odorant by the receptor protein. The vibration hypothesis predicts that OR proteins should be able to discriminate compounds containing deuterium from their common counterparts which contain hydrogen instead of deuterium. This study tests this prediction in honeybees (Apis mellifera) using the proboscis extension reflex learning in a differential conditioning paradigm. Rewarding one odour (e.g. a deuterated compound) with sucrose and not rewarding the respective analogue (e.g. hydrogen-based odorant) shows that honeybees readily learn to discriminate hydrogen-based odorants from their deuterated counterparts and supports the idea that intra-molecular vibrations may contribute to odour discrimination.

Original languageEnglish (US)
Article number20133089
JournalProceedings of the Royal Society B: Biological Sciences
Volume281
Issue number1778
DOIs
StatePublished - Jan 22 2014

Keywords

  • Differential conditioning
  • Proboscis extension reflex
  • Vibration hypothesis of olfaction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

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