Horizontal gene transfer is a significant driver of gene innovation in dinoflagellates

Jennifer H. Wisecaver, Michael L. Brosnahan, Jeremiah Hackett

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The dinoflagellates are an evolutionarily and ecologically important group of microbial eukaryotes. Previous work suggests that horizontal gene transfer (HGT) is an important source of gene innovation in these organisms. However, dinoflagellate genomes are notoriously large and complex, making genomic investigation of this phenomenon impractical with currently available sequencing technology. Fortunately, de novo transcriptome sequencing and assembly provides an alternative approach for investigating HGT. We sequenced the transcriptome of the dinoflagellate Alexandrium tamarense Group IV to investigate how HGT has contributed to gene innovation in this group. Our comprehensive A. tamarense Group IV gene set was compared with those of 16 other eukaryotic genomes. Ancestral gene content reconstruction of ortholog groups shows that A. tamarense Group IV has the largest number of gene families gained (314-1,563 depending on inference method) relative to all other organisms in the analysis (0-782). Phylogenomic analysis indicates that genes horizontally acquired from bacteria are a significant proportion of this gene influx, as are genes transferred fromother eukaryotes either through HGT or endosymbiosis. The dinoflagellates also display curious cases of gene loss associated with mitochondrial metabolism including the entire Complex I of oxidative phosphorylation. Some of these missing genes have been functionally replaced by bacterial and eukaryotic xenologs. The transcriptome of A. tamarense Group IV lends strong support to a growing body of evidence that dinoflagellate genomes are extraordinarily impacted by HGT.

Original languageEnglish (US)
Pages (from-to)2368-2381
Number of pages14
JournalGenome Biology and Evolution
Volume5
Issue number12
DOIs
StatePublished - 2013

Fingerprint

Dinoflagellida
Horizontal Gene Transfer
gene transfer
dinoflagellate
innovation
gene
Genes
genes
Transcriptome
genome
Genome
eukaryote
Eukaryota
transcriptome
eukaryotic cells
horizontal gene transfer
Miozoa
NADH dehydrogenase (ubiquinone)
Alexandrium
Symbiosis

Keywords

  • Alexandrium tamarense Group IV
  • De novo transcriptome assembly
  • Gene innovation
  • Mitochondrial metabolism
  • Phylogenetic profile
  • Phylogenomics

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Horizontal gene transfer is a significant driver of gene innovation in dinoflagellates. / Wisecaver, Jennifer H.; Brosnahan, Michael L.; Hackett, Jeremiah.

In: Genome Biology and Evolution, Vol. 5, No. 12, 2013, p. 2368-2381.

Research output: Contribution to journalArticle

Wisecaver, Jennifer H. ; Brosnahan, Michael L. ; Hackett, Jeremiah. / Horizontal gene transfer is a significant driver of gene innovation in dinoflagellates. In: Genome Biology and Evolution. 2013 ; Vol. 5, No. 12. pp. 2368-2381.
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