Hormonal regulation of pancreatic islet adenyl cyclase

W. J. Thompson, David G Johnson, R. H. Williams

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Adenyl cyclase activity of rat pancreatic islet membrane was increased by secretin, pancreozymin, and isoproterenol, while ACTH, glucagon, growth hormone, and insulin had no effect. Both secretin and isoproterenol activations were enhanced by prostaglandin E1 (PGE1) and GTP. Isoproterenol activation was additive with PGE1, as was that of secretin with PGE1, but only in the presence of GTP. Secretin activation in the presence of PGE1 and GTP was equivalent to NaF stimulation. Kinetic analysis indicated that secretin and GTP increased the maximum velocity of the adenyl cyclase and tended to decrease the apparent affinity of the enzyme for ATP. Glucagon activation of islet membrane adenyl cyclase was dependent upon prior treatment of the membrane preparation with EGTA and the use of inhibitors of proteolytic enzymes during the collagenase digestion phase of islet preparation. These results suggest that hormonal regulation of insulin secretion may be affected by PGE1 and guanine nucleotide modulation of the adenyl cyclase activation process.

Original languageEnglish (US)
Pages (from-to)1658-1663
Number of pages6
JournalBiochemistry
Volume15
Issue number8
StatePublished - 1976
Externally publishedYes

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Secretin
Alprostadil
Islets of Langerhans
Adenylyl Cyclases
Guanosine Triphosphate
Chemical activation
Isoproterenol
Glucagon
Membranes
Insulin
Guanine Nucleotides
Egtazic Acid
Cholecystokinin
Collagenases
Adrenocorticotropic Hormone
Growth Hormone
Rats
Digestion
Peptide Hydrolases
Adenosine Triphosphate

ASJC Scopus subject areas

  • Biochemistry

Cite this

Thompson, W. J., Johnson, D. G., & Williams, R. H. (1976). Hormonal regulation of pancreatic islet adenyl cyclase. Biochemistry, 15(8), 1658-1663.

Hormonal regulation of pancreatic islet adenyl cyclase. / Thompson, W. J.; Johnson, David G; Williams, R. H.

In: Biochemistry, Vol. 15, No. 8, 1976, p. 1658-1663.

Research output: Contribution to journalArticle

Thompson, WJ, Johnson, DG & Williams, RH 1976, 'Hormonal regulation of pancreatic islet adenyl cyclase', Biochemistry, vol. 15, no. 8, pp. 1658-1663.
Thompson, W. J. ; Johnson, David G ; Williams, R. H. / Hormonal regulation of pancreatic islet adenyl cyclase. In: Biochemistry. 1976 ; Vol. 15, No. 8. pp. 1658-1663.
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