Host nuclear genotype influences phenotype of a conditional mutualist symbiont

Martha S Hunter, P. Asiimwe, A. G. Himler, S. E. Kelly

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Arthropods commonly carry maternally inherited intracellular bacterial symbionts that may profoundly influence host biology and evolution. The intracellular symbiont Rickettsia sp. nr. bellii swept rapidly into populations of the sweetpotato whitefly Bemisia tabaci in the south-western USA. Previous laboratory experiments showed female-bias and fitness benefits were associated with Rickettsia infection, potentially explaining the high frequencies of infection observed in field populations, but the effects varied with whitefly genetic line. Here, we explored whether host extranuclear or nuclear genes influenced the variation in the Rickettsia–host phenotype in two genetic lines of the whitefly host, each with Rickettsia-infected and uninfected sublines. Introgression between the Rickettsia-infected subline of one genetic line and the Rickettsia-uninfected subline of the other was used to create two new sublines, each with the maternally inherited extranuclear genetic lineages of one line (Rickettsia, two other symbionts and the mitochondria) and the nuclear genotype of the other. Performance assays comparing the original and new lines showed that in addition to Rickettsia, the interaction of Rickettsia infection with host nuclear genotype influenced development time and the sex ratio of the progeny, whereas the extranuclear genotype did not. Host nuclear genotype, but not extranuclear genotype, also influenced the titre of Rickettsia. Our results support the hypothesis that differences in host nuclear genotype alone may explain considerable within-population variation in host–symbiont phenotype and may contribute to the observed variation in Rickettsia–whitefly interactions worldwide.

Original languageEnglish (US)
Pages (from-to)141-149
Number of pages9
JournalJournal of Evolutionary Biology
Volume30
Issue number1
DOIs
StatePublished - Jan 1 2017

Fingerprint

Rickettsia
symbiont
symbionts
phenotype
genotype
whitefly
genetic lines
Bemisia tabaci
Aleyrodidae
introgression
mitochondrion
infection
arthropod
sex ratio
fitness
assay
arthropods
gene
mitochondria
Biological Sciences

Keywords

  • Bemisia tabaci
  • extranuclear inheritance
  • Rickettsia bellii
  • secondary symbiont
  • sex ratio
  • sweetpotato whitefly
  • uniparental inheritance
  • Wolbachia

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Cite this

Host nuclear genotype influences phenotype of a conditional mutualist symbiont. / Hunter, Martha S; Asiimwe, P.; Himler, A. G.; Kelly, S. E.

In: Journal of Evolutionary Biology, Vol. 30, No. 1, 01.01.2017, p. 141-149.

Research output: Contribution to journalArticle

Hunter, Martha S ; Asiimwe, P. ; Himler, A. G. ; Kelly, S. E. / Host nuclear genotype influences phenotype of a conditional mutualist symbiont. In: Journal of Evolutionary Biology. 2017 ; Vol. 30, No. 1. pp. 141-149.
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