Host species-specific conservation of a family of repeated DNA sequences in the genome of a fungal plant pathogen

J. E. Hamer, L. Farrall, Marc Joel Orbach, B. Valent, F. G. Chumley

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

We have identified a family of dispersed repetitive DNA sequences in the genome of Magnaporthe grisea, the fungus that causes rice blast disease. We have named this family of DNA sequences 'MGR' for M. grisea repeat. Analysis of five MGR clones demonstrates that MGR sequences are highly polymorphic. The segregation of MGR sequences in genetic crosses and hybridization of MGR probes to separated, chromosome-size DNA molecules of M. grisea shows that this family of sequences is distributed among the M. grisea chromosomes. MGR sequences also hybridize to discrete poly(A)+ RNAs. Southern blot analysis using a MGR probe can distinguish rice pathogens from various sources. However, MGR sequences are not highly conserved in the genomes of M. grisea field isolates that do not infect rice. These results suggest that host selection for a specific pathogen genotype has occurred during the breeding and cultivation of rice.

Original languageEnglish (US)
Pages (from-to)9981-9985
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number24
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Fungal Genome
Magnaporthe
Chromosomes
Genetic Crosses
Genome
Genetic Hybridization
Interspersed Repetitive Sequences
Southern Blotting
Breeding
Fungi
Clone Cells
Genotype
Messenger RNA
Oryza
DNA

Keywords

  • evolution
  • host-pathogen
  • Magnaporthe grisea
  • Pyricularia
  • Rice blast

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Host species-specific conservation of a family of repeated DNA sequences in the genome of a fungal plant pathogen. / Hamer, J. E.; Farrall, L.; Orbach, Marc Joel; Valent, B.; Chumley, F. G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, No. 24, 1989, p. 9981-9985.

Research output: Contribution to journalArticle

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