HPLC determination of polycyclic aromatic compounds by fluorescence detected with a charge-coupled device

Rafi D. Jalkian, M Bonner Denton

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A solid-state two-dimensional charge-coupled device (CCD) is used to quantitate polycyclic aromatic compounds by native fluorescence. The mixture is separated by reverse phase high performance liquid chromatography (HPLC) and the fluorescence emission from each peak is integrated by the detector. A new technique, charge-dependent variable binning, applied to one-dimensional spectroscopy leads to outstanding dynamic range. Excellent linearity and limits of detection are obtained for several priority pollutants. Synchronous and derivative techniques can be applied to the two-dimensional fluorescence data obtained to resolve overlapping peaks. The analyzed mixture consists of fluorene, phenanthrene, anthracene, fluoranthene, pyrene, chrysene, and perylene.

Original languageEnglish (US)
Pages (from-to)91-102
Number of pages12
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume1054
DOIs
StatePublished - May 17 1989

ASJC Scopus subject areas

  • Applied Mathematics
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering
  • Computer Science Applications

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