HPV E7 viral oncoprotein disrupts transcriptional regulation of L1Md retrotransposon

Diego E. Montoya-Durango, Kenneth Ramos

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Murine L1Md-A5 retrotransposon is a redox-inducible element regulated by Nrf-2/JunD and E2F/Rb-binding sites within its promoter (5′-UTR). Because the human papillomavirus (HPV) oncoprotein E7 interacts with retinoblastoma (pRb) and members of the AP1 family, studies were conducted to examine functional interactions between HPV E7, pRb, and histone deacetylase 2 (HDAC2) in the regulation of L1Md-A5. Using a transient heterologous transcription system we found that HPV E7 alone, or in combination with HDAC2, disrupted pRb-mediated L1MdA-5 transactivation. HPV E7 also ablated the transcriptional response of L1Md-A5 to genotoxic stress, but did not interfere with basal activity. We conclude that HPV E7 associates with proteins involved in the assembly of macromolecular complexes that regulate antioxidant and E2F/Rb sites within L1MdA-5 to regulate biological activity

Original languageEnglish (US)
Pages (from-to)102-106
Number of pages5
JournalFEBS Letters
Volume586
Issue number1
DOIs
StatePublished - Jan 2 2012
Externally publishedYes

Fingerprint

Histone Deacetylase 2
Retroelements
Oncogene Proteins
Macromolecular Substances
5' Untranslated Regions
Transcription
Bioactivity
Antioxidants
Binding Sites
Retinoblastoma
Proteins
Transcriptional Activation
DNA Damage
Oxidation-Reduction

Keywords

  • 5′-Untranslated region (5′-UTR)
  • Benzo(a)pyrene (BaP)
  • E7 viral oncoprotein
  • Long interspersed nuclear element (LINE1 or L1)
  • Retinoblastoma protein family

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

HPV E7 viral oncoprotein disrupts transcriptional regulation of L1Md retrotransposon. / Montoya-Durango, Diego E.; Ramos, Kenneth.

In: FEBS Letters, Vol. 586, No. 1, 02.01.2012, p. 102-106.

Research output: Contribution to journalArticle

@article{b25c9e04cb3b4c49a6e49f2adc350569,
title = "HPV E7 viral oncoprotein disrupts transcriptional regulation of L1Md retrotransposon",
abstract = "Murine L1Md-A5 retrotransposon is a redox-inducible element regulated by Nrf-2/JunD and E2F/Rb-binding sites within its promoter (5′-UTR). Because the human papillomavirus (HPV) oncoprotein E7 interacts with retinoblastoma (pRb) and members of the AP1 family, studies were conducted to examine functional interactions between HPV E7, pRb, and histone deacetylase 2 (HDAC2) in the regulation of L1Md-A5. Using a transient heterologous transcription system we found that HPV E7 alone, or in combination with HDAC2, disrupted pRb-mediated L1MdA-5 transactivation. HPV E7 also ablated the transcriptional response of L1Md-A5 to genotoxic stress, but did not interfere with basal activity. We conclude that HPV E7 associates with proteins involved in the assembly of macromolecular complexes that regulate antioxidant and E2F/Rb sites within L1MdA-5 to regulate biological activity",
keywords = "5′-Untranslated region (5′-UTR), Benzo(a)pyrene (BaP), E7 viral oncoprotein, Long interspersed nuclear element (LINE1 or L1), Retinoblastoma protein family",
author = "Montoya-Durango, {Diego E.} and Kenneth Ramos",
year = "2012",
month = "1",
day = "2",
doi = "10.1016/j.febslet.2011.12.005",
language = "English (US)",
volume = "586",
pages = "102--106",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - HPV E7 viral oncoprotein disrupts transcriptional regulation of L1Md retrotransposon

AU - Montoya-Durango, Diego E.

AU - Ramos, Kenneth

PY - 2012/1/2

Y1 - 2012/1/2

N2 - Murine L1Md-A5 retrotransposon is a redox-inducible element regulated by Nrf-2/JunD and E2F/Rb-binding sites within its promoter (5′-UTR). Because the human papillomavirus (HPV) oncoprotein E7 interacts with retinoblastoma (pRb) and members of the AP1 family, studies were conducted to examine functional interactions between HPV E7, pRb, and histone deacetylase 2 (HDAC2) in the regulation of L1Md-A5. Using a transient heterologous transcription system we found that HPV E7 alone, or in combination with HDAC2, disrupted pRb-mediated L1MdA-5 transactivation. HPV E7 also ablated the transcriptional response of L1Md-A5 to genotoxic stress, but did not interfere with basal activity. We conclude that HPV E7 associates with proteins involved in the assembly of macromolecular complexes that regulate antioxidant and E2F/Rb sites within L1MdA-5 to regulate biological activity

AB - Murine L1Md-A5 retrotransposon is a redox-inducible element regulated by Nrf-2/JunD and E2F/Rb-binding sites within its promoter (5′-UTR). Because the human papillomavirus (HPV) oncoprotein E7 interacts with retinoblastoma (pRb) and members of the AP1 family, studies were conducted to examine functional interactions between HPV E7, pRb, and histone deacetylase 2 (HDAC2) in the regulation of L1Md-A5. Using a transient heterologous transcription system we found that HPV E7 alone, or in combination with HDAC2, disrupted pRb-mediated L1MdA-5 transactivation. HPV E7 also ablated the transcriptional response of L1Md-A5 to genotoxic stress, but did not interfere with basal activity. We conclude that HPV E7 associates with proteins involved in the assembly of macromolecular complexes that regulate antioxidant and E2F/Rb sites within L1MdA-5 to regulate biological activity

KW - 5′-Untranslated region (5′-UTR)

KW - Benzo(a)pyrene (BaP)

KW - E7 viral oncoprotein

KW - Long interspersed nuclear element (LINE1 or L1)

KW - Retinoblastoma protein family

UR - http://www.scopus.com/inward/record.url?scp=84655167540&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84655167540&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2011.12.005

DO - 10.1016/j.febslet.2011.12.005

M3 - Article

C2 - 22172279

AN - SCOPUS:84655167540

VL - 586

SP - 102

EP - 106

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1

ER -