HSP70-related proteins in bovine skeletal muscle

Vincent Guerriero, D. H. Raynes, J. A. Gutierrez

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Constitutive expression of HSP70-related proteins was detected in a variety of bovine tissues using a specific antibody. All tissues contained a 73 kilodalton protein. A lower molecular weight from (72 kilodaltons) that co-migrated on two-dimensional gels with the stressed-induced HSP70 was present in high levels in bovine skeletal muscle, but absent from rat skeletal muscle. Two-dimensional gel analysis revealed several isoforms for both the 73 and 72 kilodalton forms. Purification of HSP70-related proteins from bovine skeletal muscle, thymus gland and rat skeletal muscle demonstrated that the antibody recognized all the forms present in the tissue homogenates. The two proteins are similar but distinct as detected by one-dimensional peptide mapping. The lower molecular form was not present in fetal tissue but was detectable in newborn animals, suggesting that the levels are regulated during development.

Original languageEnglish (US)
Pages (from-to)471-477
Number of pages7
JournalJournal of Cellular Physiology
Volume140
Issue number3
StatePublished - 1989

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Muscle
Skeletal Muscle
Tissue
Rats
Proteins
Gels
Newborn Animals
Thymus
Peptide Mapping
Antibodies
Thymus Gland
Purification
Protein Isoforms
Animals
Fetus
Molecular Weight
Molecular weight
Peptides

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Guerriero, V., Raynes, D. H., & Gutierrez, J. A. (1989). HSP70-related proteins in bovine skeletal muscle. Journal of Cellular Physiology, 140(3), 471-477.

HSP70-related proteins in bovine skeletal muscle. / Guerriero, Vincent; Raynes, D. H.; Gutierrez, J. A.

In: Journal of Cellular Physiology, Vol. 140, No. 3, 1989, p. 471-477.

Research output: Contribution to journalArticle

Guerriero, V, Raynes, DH & Gutierrez, JA 1989, 'HSP70-related proteins in bovine skeletal muscle', Journal of Cellular Physiology, vol. 140, no. 3, pp. 471-477.
Guerriero, Vincent ; Raynes, D. H. ; Gutierrez, J. A. / HSP70-related proteins in bovine skeletal muscle. In: Journal of Cellular Physiology. 1989 ; Vol. 140, No. 3. pp. 471-477.
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