HspBP1, a homologue of the yeast Fes1 and Sls1 proteins, is an Hsc70 nucleotide exchange factor

Mehdi Kabani, Catherine McLellan, Deborah A. Raynes, Vincent Guerriero, Jeffrey L. Brodsky

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

The yeast FES1 and SLS1 genes encode conserved nucleotide exchange factors that act on the cytoplasmic and endoplasmic reticulum luminal Hsp70s, Ssa1p and BiP, respectively. We report here that mammalian HspBP1 is homologous to Fes1p and that HspBP1 promotes nucleotide dissociation from both Ssa1p and mammalian Hsc70. In contrast, Fes1p inefficiently strips nucleotide from mammalian Hsc70, and unlike HspBP1 does not inhibit chaperone-mediated protein refolding in vitro. Together, our data indicate that HspBP1 is a member of this new class of nucleotide exchange factors that exhibit varying degrees of compartment and species specificity.

Original languageEnglish (US)
Pages (from-to)339-342
Number of pages4
JournalFEBS Letters
Volume531
Issue number2
DOIs
StatePublished - Nov 6 2002

Fingerprint

HSC70 Heat-Shock Proteins
Yeast
Nucleotides
Yeasts
Proteins
Protein Refolding
Species Specificity
Endoplasmic Reticulum
Genes

Keywords

  • Fes1p
  • Hsc70
  • Hsp70
  • HspBP1
  • Nucleotide exchange
  • Sls1p

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

HspBP1, a homologue of the yeast Fes1 and Sls1 proteins, is an Hsc70 nucleotide exchange factor. / Kabani, Mehdi; McLellan, Catherine; Raynes, Deborah A.; Guerriero, Vincent; Brodsky, Jeffrey L.

In: FEBS Letters, Vol. 531, No. 2, 06.11.2002, p. 339-342.

Research output: Contribution to journalArticle

Kabani, Mehdi ; McLellan, Catherine ; Raynes, Deborah A. ; Guerriero, Vincent ; Brodsky, Jeffrey L. / HspBP1, a homologue of the yeast Fes1 and Sls1 proteins, is an Hsc70 nucleotide exchange factor. In: FEBS Letters. 2002 ; Vol. 531, No. 2. pp. 339-342.
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