Human δ opioid receptor: A stable cell line for functional studies of opioids

Ewa Malatynska, Yu Wang, Richard J. Knapp, Giovanna Santoro, Xaoping Li, Sue Waite, William R. Roeske, Henry I. Yamamura

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

A Human δ opioid receptor cDNA clone (pREPIO/ hDOR) was transfected into Chinese hamster ovary (CHO) cells. The stable cell line expressed a high density of δ opioid receptors (137 000 ± 21 600 receptors/cell). DPDPE inhibited 90% of the forskolin-stimulated cAMP accumulation in these cells with high potency (EC50 = 1.3 nM). This effect of DPDPE was antagonized by nal-trindole. The pseudo-pA2 value (Ke)of 155 pM for naltrin-dole is consistent with that measured for δ receptor antagonism in the mouse vas deferens. This is the first detailed characterization of DPDPE activity on forskolin-stimulated cAMP accumulation mediated through a human δ opioid receptor. The data support the use of the recombinant cell line for functional studies of opioid drugs.

Original languageEnglish (US)
Pages (from-to)613-616
Number of pages4
JournalNeuroReport
Volume6
Issue number4
DOIs
StatePublished - Mar 1995

Keywords

  • Adenylyl cyclase
  • CHO cells
  • Camp
  • DPDPE
  • Human δ opioid receptor
  • Naltrindole
  • Radioligand binding
  • Stable expression
  • Transfection

ASJC Scopus subject areas

  • Neuroscience(all)

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