Human EP3I prostanoid receptor induces VEGF and VEGF receptor-1 mRNA expression

Tomoko Taniguchi, Hiromichi Fujino, Davelene D. Israel, John W Regan, Toshihiko Murayama

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A critical event in tumor development is the formation of new blood vessels to provide oxygen, nutrients and growth factors to the rapidly growing cancer cells. This process of angiogenesis is complex, however, it is well established that vascular endothelial growth factor (VEGF)-mediated signaling is an important early event. Knockout mice studies have implicated the EP3 receptor in tumor development and angiogenesis; however, the signaling mechanism involved with this effect is unclear. We now show that stimulation of the EP3I isoform of the human EP3 receptor with prostaglandin E2 increases the mRNA expression of both VEGF and its cognate receptor VEGF receptor-1 (VEGFR-1). These inductions by the EP3I receptor involve the sequential activation of phosphatidylinositol 3-kinase and the extracellular signal-regulated kinases. Up-regulation of VEGF and VEGFR-1 mRNA by the human EP3I receptor has not been previously reported and further strengthen the role of this receptor in tumor-associated angiogenesis.

Original languageEnglish (US)
Pages (from-to)1173-1178
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume377
Issue number4
DOIs
StatePublished - Dec 26 2008

Fingerprint

Vascular Endothelial Growth Factor Receptor-1
Vascular Endothelial Growth Factor Receptor
Prostaglandins
Tumors
Messenger RNA
Vascular Endothelial Growth Factor A
Phosphatidylinositol 3-Kinase
Neoplasms
Extracellular Signal-Regulated MAP Kinases
Blood vessels
Dinoprostone
Nutrients
Intercellular Signaling Peptides and Proteins
Protein Isoforms
Chemical activation
Cells
Knockout Mice
Oxygen
Blood Vessels
Up-Regulation

Keywords

  • EP3 receptor
  • PGE
  • VEGF
  • VEGFR-1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Human EP3I prostanoid receptor induces VEGF and VEGF receptor-1 mRNA expression. / Taniguchi, Tomoko; Fujino, Hiromichi; Israel, Davelene D.; Regan, John W; Murayama, Toshihiko.

In: Biochemical and Biophysical Research Communications, Vol. 377, No. 4, 26.12.2008, p. 1173-1178.

Research output: Contribution to journalArticle

Taniguchi, Tomoko ; Fujino, Hiromichi ; Israel, Davelene D. ; Regan, John W ; Murayama, Toshihiko. / Human EP3I prostanoid receptor induces VEGF and VEGF receptor-1 mRNA expression. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 377, No. 4. pp. 1173-1178.
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