Human islet isolation for autologous transplantation: Comparison of yield and function using serva/nordmark Versus Roche Enzymes

T. Anazawa, A. N. Balamurugan, M. Bellin, H. J. Zhang, S. Matsumoto, Y. Yonekawa, T. Tanaka, G. Loganathan, K. K. Papas, G. J. Beilman, B. J. Hering, D. E.R. Sutherland

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Islet autotransplantation (IAT) is used to preserve as much insulin-secretory capacity as possible in patients undergoing total pancreatectomy for painful chronic pancreatitis. The enzyme used to dissociate the pancreas is a critical determinant of islet yield, which is correlated with posttransplant function. Here, we present our experience with IAT procedures to compare islet product data using the new enzyme SERVA/Nordmark (SN group; n = 46) with the standard enzyme Liberase-HI (LH group; n = 40). Total islet yields (mean ± standard deviation; 216 417 ± 79 278 islet equivalent [IEQ] in the LH group; 227 958 ± 58 544 IEQ in the SN group; p = 0.67) were similar. However, the percentage of embedded islets is higher in the SN group compared to the LH group. Significant differences were found in pancreas digestion time, dilution time, and digested pancreas weight between the two groups. Multivariate linear regression analysis showed the two groups differed in portal venous pressure changes. The incidence of graft function and insulin independence was not different between the two groups. The SN and LH enzymes are associated with similar outcomes for IAT. Further optimization of the collagenase/neutral protease ratio is necessary to reduce the number of embedded islets obtained when using the SN enzyme.

Original languageEnglish (US)
Pages (from-to)2383-2391
Number of pages9
JournalAmerican Journal of Transplantation
Volume9
Issue number10
DOIs
StatePublished - Oct 2009

Keywords

  • Collagenase
  • Islet autotransplantation
  • Islet isolation
  • Islet transplantation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Transplantation
  • Pharmacology (medical)

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