Human liver quality is a dominant factor in the outcome of in vitro studies

R. L. Fisher, A Jay Gandolfi, K. Brendel

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Donated human liver in the form of precision-cut tissue slices or isolated hepatocytes, is increasingly being used to predict metabolism and toxicity of xenobiotics in man. These tissue slices or hepatocytes can also be cold-preserved and cryopreserved to prolong their use for biological experiments. The viability of human liver could substantially affect the outcome of such experimentation. The goal of this investigation was to assess the viability of donated human livers, in the form of tissue slices, as they were received and to determine how varying degrees of liver quality affect experimental outcomes. Over one hundred human livers were categorized according to initial viability, as assessed by ATP content, K+ retention, protein synthesis, and LDH leakage. Each liver was placed in a low-, a medium-, or a high-quality group. The results showed that 76% of transplant-grade tissue (procured for transplantation) fell into the high-viability classification while the majority of research-grade tissue (not procured for transplantation) fell into the lowest viability classification. It was also found that only tissue slices prepared from highly viable human liver could be cold-preserved and cryopreserved. Dichlorobenzene metabolism was also greater in slices from highly viable human livers as compared to less viable livers. This study showed that human liver tissue acquired for medical research substantially varies in its viability and that these differences will affect the experimental data obtained.

Original languageEnglish (US)
Pages (from-to)179-189
Number of pages11
JournalCell Biology and Toxicology
Volume17
Issue number3
DOIs
StatePublished - 2001

Fingerprint

Liver
Tissue
Metabolism
Hepatocytes
In Vitro Techniques
Transplantation (surgical)
Tissue Transplantation
Transplants
Xenobiotics
Toxicity
Biomedical Research
Adenosine Triphosphate
Transplantation
Research

Keywords

  • Cellular viability
  • Human liver
  • Precision-cut slices

ASJC Scopus subject areas

  • Cell Biology
  • Toxicology

Cite this

Human liver quality is a dominant factor in the outcome of in vitro studies. / Fisher, R. L.; Gandolfi, A Jay; Brendel, K.

In: Cell Biology and Toxicology, Vol. 17, No. 3, 2001, p. 179-189.

Research output: Contribution to journalArticle

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