Human neutrophil activation and increased adhesion by various resuscitation fluids

Peter M Rhee, Dennis Wang, Paul Ruff, Karen Wolcott, Solenn Debraux, Brenda Austin, David Bums, Leon Sun

Research output: Contribution to journalArticle

Abstract

Introduction: Activated neutrophils play a major role in secondary tissue injury following resuscitation In trauma. Hypothesis: Human neutrophil activation and adhesion vary depending on the type of resuscitation fluid used. Methods: Whole blood from ten healthy volunteers was serially diluted in polypropylene tubes with various resuscitation fluids. Neutrophil activation (intracellular oxidative burst activity with dichloroflurodn diacetate staining) and adhesion (integrin, cell surface expression of CD18) were measured with Flow cytometry (FACS). Blood was diluted with hypertonic saline (HTS) by controlling for equal sodium content as normal saline (NS). ANOVA with Bonferoni correction for multiple comparisons was used to test for significance at the p <0.05 level. Palred t test compared to baseline at the p <0.05 levelt. Results: Results are shown as percent change compared to NS as the baseline. There was a significant dose responsive increase in neutrophils oxidative burst activity all fluids except for 25% albumin and HTS. The increase was markedly higher with crystalloids and artificial colloids. Dilution - Blood/fluid 90ul/10ul 75ul/25ul SOul/SOul 25ul/7Sul Normal Saline * 100 136† 555† 1380†* Lactated Ringers * 112 154† 362† 1261†* Dextran * 99 148† 427†1726†* Hespan * 104 128† 296† 1876†* 7.5% HTS by [Na+] 95 92 96 108 3.5% HTS by [Na+] 93 93 98 103 25% Albumin 88 91 95 100 5% Albumin * 98 118 153† 223*† There was also a similar significant increase in the CD18 expression with artificial colloids (p < 0.05) but not with albumin or crystalloids. Hypertonic saline caused a decrease in CD18 expression. Conclusions: All resuscitative fluids may not be similar nor innocuous, as demonstrated by the dose response increase in neutrophil activation and adhesion. The type of resuscitative fluid and volume used may contribute to reperfusion injury in trauma patients.

Original languageEnglish (US)
JournalCritical Care Medicine
Volume26
Issue number1 SUPPL.
StatePublished - 1998
Externally publishedYes

Fingerprint

Neutrophil Activation
Resuscitation
Albumins
Respiratory Burst
Colloids
Wounds and Injuries
Neutrophils
Hydroxyethyl Starch Derivatives
Polypropylenes
Dextrans
Reperfusion Injury
Cell Adhesion
Integrins
Analysis of Variance
Healthy Volunteers
Flow Cytometry
Sodium
Staining and Labeling
crystalloid solutions

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Rhee, P. M., Wang, D., Ruff, P., Wolcott, K., Debraux, S., Austin, B., ... Sun, L. (1998). Human neutrophil activation and increased adhesion by various resuscitation fluids. Critical Care Medicine, 26(1 SUPPL.).

Human neutrophil activation and increased adhesion by various resuscitation fluids. / Rhee, Peter M; Wang, Dennis; Ruff, Paul; Wolcott, Karen; Debraux, Solenn; Austin, Brenda; Bums, David; Sun, Leon.

In: Critical Care Medicine, Vol. 26, No. 1 SUPPL., 1998.

Research output: Contribution to journalArticle

Rhee, PM, Wang, D, Ruff, P, Wolcott, K, Debraux, S, Austin, B, Bums, D & Sun, L 1998, 'Human neutrophil activation and increased adhesion by various resuscitation fluids', Critical Care Medicine, vol. 26, no. 1 SUPPL..
Rhee PM, Wang D, Ruff P, Wolcott K, Debraux S, Austin B et al. Human neutrophil activation and increased adhesion by various resuscitation fluids. Critical Care Medicine. 1998;26(1 SUPPL.).
Rhee, Peter M ; Wang, Dennis ; Ruff, Paul ; Wolcott, Karen ; Debraux, Solenn ; Austin, Brenda ; Bums, David ; Sun, Leon. / Human neutrophil activation and increased adhesion by various resuscitation fluids. In: Critical Care Medicine. 1998 ; Vol. 26, No. 1 SUPPL.
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abstract = "Introduction: Activated neutrophils play a major role in secondary tissue injury following resuscitation In trauma. Hypothesis: Human neutrophil activation and adhesion vary depending on the type of resuscitation fluid used. Methods: Whole blood from ten healthy volunteers was serially diluted in polypropylene tubes with various resuscitation fluids. Neutrophil activation (intracellular oxidative burst activity with dichloroflurodn diacetate staining) and adhesion (integrin, cell surface expression of CD18) were measured with Flow cytometry (FACS). Blood was diluted with hypertonic saline (HTS) by controlling for equal sodium content as normal saline (NS). ANOVA with Bonferoni correction for multiple comparisons was used to test for significance at the p <0.05 level. Palred t test compared to baseline at the p <0.05 levelt. Results: Results are shown as percent change compared to NS as the baseline. There was a significant dose responsive increase in neutrophils oxidative burst activity all fluids except for 25{\%} albumin and HTS. The increase was markedly higher with crystalloids and artificial colloids. Dilution - Blood/fluid 90ul/10ul 75ul/25ul SOul/SOul 25ul/7Sul Normal Saline * 100 136† 555† 1380†* Lactated Ringers * 112 154† 362† 1261†* Dextran * 99 148† 427†1726†* Hespan * 104 128† 296† 1876†* 7.5{\%} HTS by [Na+] 95 92 96 108 3.5{\%} HTS by [Na+] 93 93 98 103 25{\%} Albumin 88 91 95 100 5{\%} Albumin * 98 118 153† 223*† There was also a similar significant increase in the CD18 expression with artificial colloids (p < 0.05) but not with albumin or crystalloids. Hypertonic saline caused a decrease in CD18 expression. Conclusions: All resuscitative fluids may not be similar nor innocuous, as demonstrated by the dose response increase in neutrophil activation and adhesion. The type of resuscitative fluid and volume used may contribute to reperfusion injury in trauma patients.",
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AU - Rhee, Peter M

AU - Wang, Dennis

AU - Ruff, Paul

AU - Wolcott, Karen

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AU - Austin, Brenda

AU - Bums, David

AU - Sun, Leon

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N2 - Introduction: Activated neutrophils play a major role in secondary tissue injury following resuscitation In trauma. Hypothesis: Human neutrophil activation and adhesion vary depending on the type of resuscitation fluid used. Methods: Whole blood from ten healthy volunteers was serially diluted in polypropylene tubes with various resuscitation fluids. Neutrophil activation (intracellular oxidative burst activity with dichloroflurodn diacetate staining) and adhesion (integrin, cell surface expression of CD18) were measured with Flow cytometry (FACS). Blood was diluted with hypertonic saline (HTS) by controlling for equal sodium content as normal saline (NS). ANOVA with Bonferoni correction for multiple comparisons was used to test for significance at the p <0.05 level. Palred t test compared to baseline at the p <0.05 levelt. Results: Results are shown as percent change compared to NS as the baseline. There was a significant dose responsive increase in neutrophils oxidative burst activity all fluids except for 25% albumin and HTS. The increase was markedly higher with crystalloids and artificial colloids. Dilution - Blood/fluid 90ul/10ul 75ul/25ul SOul/SOul 25ul/7Sul Normal Saline * 100 136† 555† 1380†* Lactated Ringers * 112 154† 362† 1261†* Dextran * 99 148† 427†1726†* Hespan * 104 128† 296† 1876†* 7.5% HTS by [Na+] 95 92 96 108 3.5% HTS by [Na+] 93 93 98 103 25% Albumin 88 91 95 100 5% Albumin * 98 118 153† 223*† There was also a similar significant increase in the CD18 expression with artificial colloids (p < 0.05) but not with albumin or crystalloids. Hypertonic saline caused a decrease in CD18 expression. Conclusions: All resuscitative fluids may not be similar nor innocuous, as demonstrated by the dose response increase in neutrophil activation and adhesion. The type of resuscitative fluid and volume used may contribute to reperfusion injury in trauma patients.

AB - Introduction: Activated neutrophils play a major role in secondary tissue injury following resuscitation In trauma. Hypothesis: Human neutrophil activation and adhesion vary depending on the type of resuscitation fluid used. Methods: Whole blood from ten healthy volunteers was serially diluted in polypropylene tubes with various resuscitation fluids. Neutrophil activation (intracellular oxidative burst activity with dichloroflurodn diacetate staining) and adhesion (integrin, cell surface expression of CD18) were measured with Flow cytometry (FACS). Blood was diluted with hypertonic saline (HTS) by controlling for equal sodium content as normal saline (NS). ANOVA with Bonferoni correction for multiple comparisons was used to test for significance at the p <0.05 level. Palred t test compared to baseline at the p <0.05 levelt. Results: Results are shown as percent change compared to NS as the baseline. There was a significant dose responsive increase in neutrophils oxidative burst activity all fluids except for 25% albumin and HTS. The increase was markedly higher with crystalloids and artificial colloids. Dilution - Blood/fluid 90ul/10ul 75ul/25ul SOul/SOul 25ul/7Sul Normal Saline * 100 136† 555† 1380†* Lactated Ringers * 112 154† 362† 1261†* Dextran * 99 148† 427†1726†* Hespan * 104 128† 296† 1876†* 7.5% HTS by [Na+] 95 92 96 108 3.5% HTS by [Na+] 93 93 98 103 25% Albumin 88 91 95 100 5% Albumin * 98 118 153† 223*† There was also a similar significant increase in the CD18 expression with artificial colloids (p < 0.05) but not with albumin or crystalloids. Hypertonic saline caused a decrease in CD18 expression. Conclusions: All resuscitative fluids may not be similar nor innocuous, as demonstrated by the dose response increase in neutrophil activation and adhesion. The type of resuscitative fluid and volume used may contribute to reperfusion injury in trauma patients.

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