human papillomavirus infection in "young" versus "old" patients with squamous cell carcinoma of the head and neck

Elizabeth A. Sisk, Carol R. Bradford, Abraham Jacob, Christopher H. Yian, Kim M. Staton, Gong Tang, Monte O. Harris, Thomas E. Carey, Wayne D. Lancaster, Lucie Gregoire

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Background. Human papillomavirus (HPV) represents a potential risk factor for squamous cell cancer of the head and neck (SCCHN). We evaluated the prevalence of HPV DNA in patients with SCCHN diagnosed at the University of Michigan from 1994-1996. Methods. Patients were stratified by age at diagnosis as "young" (<50 years; median, 39) or "old" (>50 years; median, 66). Fourteen "young" and 14 "old" were matched for tumor site, and 4 additional "old" patients were included. Specimens were analyzed by polymerase chain reaction for HPV DNA using 2 sets of consensus primers. HPV sequences were confirmed by Southern blot hybridization and typed with type-specific probes. Results. Overall, 15 of 32 (46.9%) samples contained HPV sequences. HPV 16 was detected in 9 of 15 (60%), HPV-18 in 1 of 15 (6.6%), and 5 of 15 (33.3%) remained untyped by multiple methods, When stratified, 7 of 14 (50%) "young" were HPV-positive compared with 8 of 18 (44.4%) "old" (p = .76). Survival in patients with HPV-positive SCCHN was significantly longer than that for HPV-negative patients. Conclusions. The incidence of HPV in "young" versus "old" is not significantly different, suggesting similar roles for both groups. Patients with HPV-positive tumors may have a survival advantage relative to patients with HPV-negative tumors.

Original languageEnglish (US)
Pages (from-to)649-657
Number of pages9
JournalHead and Neck
Volume22
Issue number7
StatePublished - Oct 2000
Externally publishedYes

Fingerprint

Papillomavirus Infections
Squamous Cell Neoplasms
Head and Neck Neoplasms
Carcinoma, squamous cell of head and neck
Human papillomavirus 18
Neoplasms
Survival
Human papillomavirus 16
DNA
Southern Blotting

Keywords

  • Head and neck human papillomavirus
  • Neoplasms
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Sisk, E. A., Bradford, C. R., Jacob, A., Yian, C. H., Staton, K. M., Tang, G., ... Gregoire, L. (2000). human papillomavirus infection in "young" versus "old" patients with squamous cell carcinoma of the head and neck. Head and Neck, 22(7), 649-657.

human papillomavirus infection in "young" versus "old" patients with squamous cell carcinoma of the head and neck. / Sisk, Elizabeth A.; Bradford, Carol R.; Jacob, Abraham; Yian, Christopher H.; Staton, Kim M.; Tang, Gong; Harris, Monte O.; Carey, Thomas E.; Lancaster, Wayne D.; Gregoire, Lucie.

In: Head and Neck, Vol. 22, No. 7, 10.2000, p. 649-657.

Research output: Contribution to journalArticle

Sisk, EA, Bradford, CR, Jacob, A, Yian, CH, Staton, KM, Tang, G, Harris, MO, Carey, TE, Lancaster, WD & Gregoire, L 2000, 'human papillomavirus infection in "young" versus "old" patients with squamous cell carcinoma of the head and neck', Head and Neck, vol. 22, no. 7, pp. 649-657.
Sisk, Elizabeth A. ; Bradford, Carol R. ; Jacob, Abraham ; Yian, Christopher H. ; Staton, Kim M. ; Tang, Gong ; Harris, Monte O. ; Carey, Thomas E. ; Lancaster, Wayne D. ; Gregoire, Lucie. / human papillomavirus infection in "young" versus "old" patients with squamous cell carcinoma of the head and neck. In: Head and Neck. 2000 ; Vol. 22, No. 7. pp. 649-657.
@article{38d8cee24a0c435791be256b4f0c42f2,
title = "human papillomavirus infection in {"}young{"} versus {"}old{"} patients with squamous cell carcinoma of the head and neck",
abstract = "Background. Human papillomavirus (HPV) represents a potential risk factor for squamous cell cancer of the head and neck (SCCHN). We evaluated the prevalence of HPV DNA in patients with SCCHN diagnosed at the University of Michigan from 1994-1996. Methods. Patients were stratified by age at diagnosis as {"}young{"} (<50 years; median, 39) or {"}old{"} (>50 years; median, 66). Fourteen {"}young{"} and 14 {"}old{"} were matched for tumor site, and 4 additional {"}old{"} patients were included. Specimens were analyzed by polymerase chain reaction for HPV DNA using 2 sets of consensus primers. HPV sequences were confirmed by Southern blot hybridization and typed with type-specific probes. Results. Overall, 15 of 32 (46.9{\%}) samples contained HPV sequences. HPV 16 was detected in 9 of 15 (60{\%}), HPV-18 in 1 of 15 (6.6{\%}), and 5 of 15 (33.3{\%}) remained untyped by multiple methods, When stratified, 7 of 14 (50{\%}) {"}young{"} were HPV-positive compared with 8 of 18 (44.4{\%}) {"}old{"} (p = .76). Survival in patients with HPV-positive SCCHN was significantly longer than that for HPV-negative patients. Conclusions. The incidence of HPV in {"}young{"} versus {"}old{"} is not significantly different, suggesting similar roles for both groups. Patients with HPV-positive tumors may have a survival advantage relative to patients with HPV-negative tumors.",
keywords = "Head and neck human papillomavirus, Neoplasms, Squamous cell carcinoma",
author = "Sisk, {Elizabeth A.} and Bradford, {Carol R.} and Abraham Jacob and Yian, {Christopher H.} and Staton, {Kim M.} and Gong Tang and Harris, {Monte O.} and Carey, {Thomas E.} and Lancaster, {Wayne D.} and Lucie Gregoire",
year = "2000",
month = "10",
language = "English (US)",
volume = "22",
pages = "649--657",
journal = "Head and Neck",
issn = "1043-3074",
publisher = "John Wiley and Sons Inc.",
number = "7",

}

TY - JOUR

T1 - human papillomavirus infection in "young" versus "old" patients with squamous cell carcinoma of the head and neck

AU - Sisk, Elizabeth A.

AU - Bradford, Carol R.

AU - Jacob, Abraham

AU - Yian, Christopher H.

AU - Staton, Kim M.

AU - Tang, Gong

AU - Harris, Monte O.

AU - Carey, Thomas E.

AU - Lancaster, Wayne D.

AU - Gregoire, Lucie

PY - 2000/10

Y1 - 2000/10

N2 - Background. Human papillomavirus (HPV) represents a potential risk factor for squamous cell cancer of the head and neck (SCCHN). We evaluated the prevalence of HPV DNA in patients with SCCHN diagnosed at the University of Michigan from 1994-1996. Methods. Patients were stratified by age at diagnosis as "young" (<50 years; median, 39) or "old" (>50 years; median, 66). Fourteen "young" and 14 "old" were matched for tumor site, and 4 additional "old" patients were included. Specimens were analyzed by polymerase chain reaction for HPV DNA using 2 sets of consensus primers. HPV sequences were confirmed by Southern blot hybridization and typed with type-specific probes. Results. Overall, 15 of 32 (46.9%) samples contained HPV sequences. HPV 16 was detected in 9 of 15 (60%), HPV-18 in 1 of 15 (6.6%), and 5 of 15 (33.3%) remained untyped by multiple methods, When stratified, 7 of 14 (50%) "young" were HPV-positive compared with 8 of 18 (44.4%) "old" (p = .76). Survival in patients with HPV-positive SCCHN was significantly longer than that for HPV-negative patients. Conclusions. The incidence of HPV in "young" versus "old" is not significantly different, suggesting similar roles for both groups. Patients with HPV-positive tumors may have a survival advantage relative to patients with HPV-negative tumors.

AB - Background. Human papillomavirus (HPV) represents a potential risk factor for squamous cell cancer of the head and neck (SCCHN). We evaluated the prevalence of HPV DNA in patients with SCCHN diagnosed at the University of Michigan from 1994-1996. Methods. Patients were stratified by age at diagnosis as "young" (<50 years; median, 39) or "old" (>50 years; median, 66). Fourteen "young" and 14 "old" were matched for tumor site, and 4 additional "old" patients were included. Specimens were analyzed by polymerase chain reaction for HPV DNA using 2 sets of consensus primers. HPV sequences were confirmed by Southern blot hybridization and typed with type-specific probes. Results. Overall, 15 of 32 (46.9%) samples contained HPV sequences. HPV 16 was detected in 9 of 15 (60%), HPV-18 in 1 of 15 (6.6%), and 5 of 15 (33.3%) remained untyped by multiple methods, When stratified, 7 of 14 (50%) "young" were HPV-positive compared with 8 of 18 (44.4%) "old" (p = .76). Survival in patients with HPV-positive SCCHN was significantly longer than that for HPV-negative patients. Conclusions. The incidence of HPV in "young" versus "old" is not significantly different, suggesting similar roles for both groups. Patients with HPV-positive tumors may have a survival advantage relative to patients with HPV-negative tumors.

KW - Head and neck human papillomavirus

KW - Neoplasms

KW - Squamous cell carcinoma

UR - http://www.scopus.com/inward/record.url?scp=0034295022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034295022&partnerID=8YFLogxK

M3 - Article

C2 - 11002318

AN - SCOPUS:0034295022

VL - 22

SP - 649

EP - 657

JO - Head and Neck

JF - Head and Neck

SN - 1043-3074

IS - 7

ER -