Human Papillomavirus L2 Facilitates Viral Escape from Late Endosomes via Sorting Nexin 17

Martina Bergant Marušič, Michelle A. Ozbun, Samuel K Campos, Michael P. Myers, Lawrence Banks

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The human papillomavirus (HPV) L2 capsid protein plays an essential role during the early stages of viral infection, but the molecular mechanisms underlying its mode of action remain obscure. Using a proteomic approach, we have identified the adaptor protein, sorting nexin 17 (SNX17) as a strong interacting partner of HPV L2. This interaction occurs through a highly conserved SNX17 consensus binding motif, which is present in the majority of HPV L2 proteins analysed. Using mutants of L2 defective for SNX17 interaction, or siRNA ablation of SNX17 expression, we demonstrate that the interaction between L2 and SNX17 is essential for viral infection. Furthermore, loss of the L2-SNX17 interaction results in enhanced turnover of the L2 protein and decreased stability of the viral capsids, and concomitantly, there is a dramatic decrease in the efficiency with which viral genomes transit to the nucleus. Indeed, using a range of endosomal and lysosomal markers, we show that capsids defective in their capacity to bind SNX17 transit much more rapidly to the lysosomal compartment. These results demonstrate that the L2-SNX17 interaction is essential for viral infection and facilitates the escape of the L2-DNA complex from the late endosomal/lysosomal compartments.

Original languageEnglish (US)
Pages (from-to)455-467
Number of pages13
JournalTraffic
Volume13
Issue number3
DOIs
StatePublished - Mar 2012

Fingerprint

Sorting Nexins
Endosomes
Virus Diseases
Capsid
Proteins
Protein Stability
Viral Genome
Capsid Proteins
Protein Transport
Ablation
Proteomics
Small Interfering RNA
Genes

Keywords

  • Endosomes
  • HPV
  • L2
  • Sorting nexin
  • Viral infection

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Structural Biology
  • Molecular Biology
  • Genetics

Cite this

Human Papillomavirus L2 Facilitates Viral Escape from Late Endosomes via Sorting Nexin 17. / Bergant Marušič, Martina; Ozbun, Michelle A.; Campos, Samuel K; Myers, Michael P.; Banks, Lawrence.

In: Traffic, Vol. 13, No. 3, 03.2012, p. 455-467.

Research output: Contribution to journalArticle

Bergant Marušič, Martina ; Ozbun, Michelle A. ; Campos, Samuel K ; Myers, Michael P. ; Banks, Lawrence. / Human Papillomavirus L2 Facilitates Viral Escape from Late Endosomes via Sorting Nexin 17. In: Traffic. 2012 ; Vol. 13, No. 3. pp. 455-467.
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