Human papillomavirus type 31 uses a caveolin 1- and dynamin 2-mediated entry pathway for infection of human keratinocytes

Jessica L. Smith, Samuel K Campos, Michelle A. Ozbun

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Papillomaviruses are species-specific and epitheliotropic DNA viruses that cause tumors in their natural hosts. Certain infections with genital human papillomavirus (HPV) types are causally related to cervical cancer development. Most papillomaviruses are thought to infect cells via a clathrin-dependent pathway, yet no studies have determined the entry route in permissive host epithelial cells. Employing fluorescently labeled and native virions, we tested the effects of dominant-negative and biochemical inhibitors of cellular endocytosis pathways. Infections of human keratinocytes, a natural host cell type for HPVs, were assessed visually and by infectious entry assays. We found that HPV type 31 (HPV31) entry and initiation of early infection events require both caveolin 1 and dynamin 2 and occur independently of clathrin-mediated endocytosis. Treatment with chlorpromazine and filipin had opposing effects on HPV31 and HPV16 infection. HPV31 entry was remarkably slow, with a half-time of ≈14 h, whereas the entry half-time of HPV16 was 4 h. Consistent with a caveola-mediated entry pathway for HPV31, the virions associated with detergent-resistant lipid rafts. During a 16-h microscopic tracking of HPV31 and HPV16 virions, no colocalization of the two viral types was observed. These data suggest that HPV31 and HPV16 virions use distinct routes for host epithelial cell entry.

Original languageEnglish (US)
Pages (from-to)9922-9931
Number of pages10
JournalJournal of Virology
Volume81
Issue number18
DOIs
StatePublished - Sep 2007
Externally publishedYes

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Caveolin 2
Dynamin II
Human papillomavirus 31
Caveolin 1
Papillomaviridae
keratinocytes
Keratinocytes
virion
clathrin
Virion
endocytosis
Infection
infection
Clathrin
epithelial cells
filipin
Endocytosis
chlorpromazine
DNA viruses
uterine cervical neoplasms

ASJC Scopus subject areas

  • Immunology

Cite this

Human papillomavirus type 31 uses a caveolin 1- and dynamin 2-mediated entry pathway for infection of human keratinocytes. / Smith, Jessica L.; Campos, Samuel K; Ozbun, Michelle A.

In: Journal of Virology, Vol. 81, No. 18, 09.2007, p. 9922-9931.

Research output: Contribution to journalArticle

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