Human peritoneal monocytic cells: Lipoprotein uptake and foam cell formation

Joy J. Winzerling, Zeinab E. Jouni, Donald J. McNamara

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Human peritoneal cells isolated from dialysis effluent have in vivo maturated human macrophages that could serve as a model for studying lipoprotein metabolism and foam cell formation. We previously characterized the low density lipoprotein (LDL) and acetylated LDL (acetyl-LDL) receptor activities of human total peritoneal cells. Now, we provide evidence that both LDL and acetyl-LDL stimulate acylCoA cholesterol:acyl transferase (ACAT) activity of peritoneal cells. Prolonged incubation of cells with LDL results in suppression of ACAT activity, while incubation with acetyl-LDL results in elevated and sustained enzyme activity. When human peritoneal cells were analyzed using flow cytometry, the cell population showed reactivity for CD2, CD4, CD8, CD20, CD14 and HLA-DR antigens. Purified human peritoneal mononuclear cells degraded LDL. Human peritoneal macrophages formed foam cells when exposed to LDL or acetyl-LDL in culture, and lipid deposition increased with incubation time. Macrophages incubated in the presence of butylated hydroxy toluene and LDL did not form foam cells.

Original languageEnglish (US)
Pages (from-to)501-513
Number of pages13
JournalLife Sciences
Volume62
Issue number6
DOIs
StatePublished - Jan 2 1998

Keywords

  • Acetylated low density lipoprotein
  • Chronic ambulatory peritoneal dialysis
  • Flow cytometry
  • Foam cells
  • Human peritoneal cells
  • Low density lipoprotein
  • Scavenger receptor

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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