Human platelet calcium measurements. Methodological considerations and comparisons with calcium mobilization in vascular smooth muscle cells

Paul R Standley, S. Gangasani, R. Prakash, M. B. Zemel, J. R. Sowers

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Platelets are used as models for vascular smooth muscle cells (VSMC) in evaluating intracellular calcium ([Ca2+](i)) metabolism in humans. This study was designed to determine if agonist-induced increases in [Ca2+](i) in platelets occur via release from intracellular stores as previously demonstrated for VSMC. Incubation of purified platelets loaded with fura-2-AM in media containing 1.5 mmol/L Ca2+ resulted in higher basal [Ca2+](i) than platelets incubated in Ca2+-free media. In addition, vasopressin-induced platelet [Ca2+](i) transients were almost completely blocked by Ca2+ channel blockers. Thus, in contrast to VSMC, the transmembranous flux of extracellular Ca2+ is the major mechanism in vasopressin-induced increases in platelet [Ca2+](i), while mobilization of intracellular Ca2+ stores is only minimally involved.

Original languageEnglish (US)
Pages (from-to)546-549
Number of pages4
JournalAmerican Journal of Hypertension
Volume4
Issue number6
StatePublished - 1991
Externally publishedYes

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Vascular Smooth Muscle
Smooth Muscle Myocytes
Blood Platelets
Calcium
Vasopressins
Fura-2

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Human platelet calcium measurements. Methodological considerations and comparisons with calcium mobilization in vascular smooth muscle cells. / Standley, Paul R; Gangasani, S.; Prakash, R.; Zemel, M. B.; Sowers, J. R.

In: American Journal of Hypertension, Vol. 4, No. 6, 1991, p. 546-549.

Research output: Contribution to journalArticle

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