Human telomeric G-quadruplex structures and G-quadruplex-interactive compounds

Clement Lin, Danzhou Yang

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Scopus citations

Abstract

G-quadruplexes are noncanonical secondary structures formed in DNA sequences containing consecutive runs of guanines. It has been shown that the 3′ G-rich single-stranded overhangs of human telomeres can form G-quadruplex structures, and the human telomeric DNA G-quadruplexes are considered attractive targets for anticancer drugs. G-quadruplex-interactive compounds have been shown to inhibit telomerase access as well as telomere capping. Nuclear magnetic resonance (NMR) spectroscopy is a powerful method in determining the G-quadruplex structures under physiologically relevant conditions. We present the NMR and biophysical methodology used in our research group for the study of G-quadruplex structures in physiologically relevant solution and their interactions with small-molecule compounds.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages171-196
Number of pages26
Volume1587
DOIs
StatePublished - 2017
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1587
ISSN (Print)10643745

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Keywords

  • Anticancer drug targets
  • G-Quadruplex structures
  • G-Quadruplex-interactive compounds
  • Human telomeres
  • Structure polymorphism
  • Telomerase inhibitors

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Lin, C., & Yang, D. (2017). Human telomeric G-quadruplex structures and G-quadruplex-interactive compounds. In Methods in Molecular Biology (Vol. 1587, pp. 171-196). (Methods in Molecular Biology; Vol. 1587). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6892-3_17