Human trophoblast noncoding RNA suppresses CIITA promoter III activity in murine B-lymphocytes

Arnar Geirsson, Raymond J. Lynch, Indu Paliwal, Alfred L.M. Bothwell, Graeme L. Hammond

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Trophoblasts lack expression of all classical MHC antigens. Determination of the mechanism involved could provide insight into selective gene suppression and allograft tolerance. Suppression of class II expression in trophoblasts is secondary to dominant negative trans-acting factors that suppress class II transactivator (CIITA) transcription. We recently described a trophoblast-derived noncoding RNA (TncRNA) that suppresses class II expression. Murine B-cells CH27 were stably transfected with TncRNA and analyzed for MHC class II and CIITA expression by FACS and Northern blots. Functional assessment of CIITA promoter III (pIII) was performed by transient transfection of promoter-reporter constructs. Methylation of pIII was assessed by Southern blots and FACS. TncRNA suppressed constitutive I-Ak and CIITA expression in murine B-cells CH27. The mechanism involves inhibition of CIITA pIII activity. The mechanism does not involve methylation of the promoter.

Original languageEnglish (US)
Pages (from-to)718-724
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume301
Issue number3
DOIs
StatePublished - Feb 14 2003
Externally publishedYes

Keywords

  • CIITA
  • Gene regulation
  • MHC class II
  • Noncoding RNA
  • Repressor
  • Xenograft

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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