Human Viperin Causes Radical SAM-Dependent Elongation of Escherichia coli, Hinting at Its Physiological Role

Micah T. Nelp, Anthony P. Young, Branden M. Stepanski, Vahe Bandarian

Research output: Research - peer-reviewArticle

Abstract

Viperin (virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible) is a widely distributed protein that is expressed in response to infection and causes antiviral effects against a broad spectrum of viruses. Viperin is a member of the radical S-adenosyl-l-methionine (SAM) superfamily of enzymes, which typically employ a 4Fe-4S cluster to reductively cleave SAM to initiate chemistry. Though the specific reaction catalyzed by viperin remains unknown, it has been shown that expression of viperin causes an increase in the fluidity of lipid membranes, which impedes the budding of nascent viral particles from the membrane inhibiting propagation of the infection. Herein, we show that expression of the human viperin homologue induces a dramatically elongated morphology of the host Escherichia coli cells. Mutation of an essential cysteine that coordinates the radical SAM cluster abrogates this effect. Thus, the native radical SAM activity of viperin is likely occurring in the host bacteria, indicating the elusive substrate is shared between both bacteria and humans, significantly narrowing the range of potential candidate substrates and providing a convenient bacterial platform from which future studies can occur.

LanguageEnglish (US)
Pages3874-3876
Number of pages3
JournalBiochemistry
Volume56
Issue number30
DOIs
StatePublished - Aug 1 2017
Externally publishedYes

Fingerprint

Methionine
Escherichia coli
Elongation
Viruses
Bacteria
Infection
Proteins
Membrane Fluidity
Membrane Lipids
Endoplasmic Reticulum
Virion
Interferons
Antiviral Agents
Cysteine
Mutation
Membranes
Enzymes
Fluidity

ASJC Scopus subject areas

  • Biochemistry

Cite this

Human Viperin Causes Radical SAM-Dependent Elongation of Escherichia coli, Hinting at Its Physiological Role. / Nelp, Micah T.; Young, Anthony P.; Stepanski, Branden M.; Bandarian, Vahe.

In: Biochemistry, Vol. 56, No. 30, 01.08.2017, p. 3874-3876.

Research output: Research - peer-reviewArticle

Nelp, Micah T. ; Young, Anthony P. ; Stepanski, Branden M. ; Bandarian, Vahe. / Human Viperin Causes Radical SAM-Dependent Elongation of Escherichia coli, Hinting at Its Physiological Role. In: Biochemistry. 2017 ; Vol. 56, No. 30. pp. 3874-3876
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