HYD1-induced increase in reactive oxygen species leads to autophagy and necrotic cell death in multiple myeloma cells

Rajesh R. Nair, Michael F. Emmons, Anne E Cress, Raul F. Argilagos, Kit Lam, William T. Kerr, Hong Gong Wang, William S. Dalton, Lori A. Hazlehurst

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

HYD1 is a D-amino acid peptide that was previously shown to inhibit adhesion of prostate cancer cells to the extracellular matrix. In this study, we show that in addition to inhibiting adhesion of multiple myeloma (MM) cells to fibronectin, HYD1 induces cell death in MM cells as a single agent. HYD1-induced cell death was necrotic in nature as shown by: (a) decrease in mitochondrial membrane potential (ΔΨm), (b) loss of total cellular ATP, and (c) increase in reactive oxygen species (ROS) production. Moreover, HYD1 treatment does not result in apoptotic cell death because it did not trigger the activation of caspases or the release of apoptosis-inducing factor and endonuclease G from the mitochondria, nor did it induce double-stranded DNA breaks. HYD1 did initiate autophagy in cells; however, autophagy was found to be an adaptive response contributing to cell survival rather than the cause of cell death. We were further able to show that N-acetyl-L-cysteine, a thiol-containing free radical scavenger, partially protects MM cells from HYD1-induced death. Additionally, N-acetyl-L-cysteine blocked HYD1-induced as well as basal levels of autophagy, suggesting that ROS can potentially trigger both cell death and cell survival pathways. Taken together, our data describe an important role of ROS in HYD1-induced necrotic cell death in MM cells.

Original languageEnglish (US)
Pages (from-to)2441-2451
Number of pages11
JournalMolecular Cancer Therapeutics
Volume8
Issue number8
DOIs
StatePublished - Aug 1 2009

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Autophagy
Multiple Myeloma
Reactive Oxygen Species
Cell Death
Acetylcysteine
Cell Survival
Apoptosis Inducing Factor
Free Radical Scavengers
Double-Stranded DNA Breaks
Mitochondrial Membrane Potential
Caspases
Fibronectins
Sulfhydryl Compounds
Extracellular Matrix
Cause of Death
Prostatic Neoplasms
Mitochondria
Adenosine Triphosphate
Amino Acids
Peptides

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

HYD1-induced increase in reactive oxygen species leads to autophagy and necrotic cell death in multiple myeloma cells. / Nair, Rajesh R.; Emmons, Michael F.; Cress, Anne E; Argilagos, Raul F.; Lam, Kit; Kerr, William T.; Wang, Hong Gong; Dalton, William S.; Hazlehurst, Lori A.

In: Molecular Cancer Therapeutics, Vol. 8, No. 8, 01.08.2009, p. 2441-2451.

Research output: Contribution to journalArticle

Nair, RR, Emmons, MF, Cress, AE, Argilagos, RF, Lam, K, Kerr, WT, Wang, HG, Dalton, WS & Hazlehurst, LA 2009, 'HYD1-induced increase in reactive oxygen species leads to autophagy and necrotic cell death in multiple myeloma cells', Molecular Cancer Therapeutics, vol. 8, no. 8, pp. 2441-2451. https://doi.org/10.1158/1535-7163.MCT-09-0113
Nair, Rajesh R. ; Emmons, Michael F. ; Cress, Anne E ; Argilagos, Raul F. ; Lam, Kit ; Kerr, William T. ; Wang, Hong Gong ; Dalton, William S. ; Hazlehurst, Lori A. / HYD1-induced increase in reactive oxygen species leads to autophagy and necrotic cell death in multiple myeloma cells. In: Molecular Cancer Therapeutics. 2009 ; Vol. 8, No. 8. pp. 2441-2451.
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