Hydration and removal of supported phospholipid films in aqueous surfactant solutions

M. T. Colberg, K. Carnes, A. E. Sáez, C. S. Grant, K. Hutchinson, D. Hesterberg

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Dynamic studies of the hydration and removal of phospholipid films attached to solid substrates were performed. The phospholipids used were 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine (DPPE), and a modification of DPPE containing a fluorescent molecular probe: n-(5-fluoresceinthicarbamoyl)-1,2-dipalmitoyl-sn-glycero-3- phosphatidylethanolamine, triethylammonium salt (FITC-DPPE). The phospholipid films were exposed to water and aqueous solutions of the anionic surfactant sodium dodecyl sulfate (SDS). The film mass was determined as a function of time by means of a quartz crystal microbalance (QCM). The crystalline structure of the film during the hydration process was analyzed by means of wide-angle X-ray diffraction. At low surfactant concentrations (below 20% of the critical micelle concentration (CMC)), the presence of surfactant increased the hydration rate of the film, as well as its maximum water uptake. At surfactant concentrations as low as 50% of the CMC, competitive hydration and removal of the phospholipid film were observed. X-Ray diffraction measurements show that the crystal structure of the DPPE films did not change significantly upon exposure to water and surfactant solutions. I n contrast, FITC-DPPE films exhibited changes in the long-range spacing of their crystalline structure upon hydration.

Original languageEnglish (US)
Pages (from-to)247-251
Number of pages5
JournalThin Solid Films
Volume327-329
Issue number1-2
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

Keywords

  • Hydration
  • Phospholipid
  • Quartz crystal microbalance
  • Surfactant

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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