Hydration and removal of supported phospholipid films in aqueous surfactant solutions

M. T. Colberg, K. Carnes, Avelino E Saez, C. S. Grant, K. Hutchinson, D. Hesterberg

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Dynamic studies of the hydration and removal of phospholipid films attached to solid substrates were performed. The phospholipids used were 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine (DPPE), and a modification of DPPE containing a fluorescent molecular probe: n-(5-fluoresceinthicarbamoyl)-1,2-dipalmitoyl-sn-glycero-3- phosphatidylethanolamine, triethylammonium salt (FITC-DPPE). The phospholipid films were exposed to water and aqueous solutions of the anionic surfactant sodium dodecyl sulfate (SDS). The film mass was determined as a function of time by means of a quartz crystal microbalance (QCM). The crystalline structure of the film during the hydration process was analyzed by means of wide-angle X-ray diffraction. At low surfactant concentrations (below 20% of the critical micelle concentration (CMC)), the presence of surfactant increased the hydration rate of the film, as well as its maximum water uptake. At surfactant concentrations as low as 50% of the CMC, competitive hydration and removal of the phospholipid film were observed. X-Ray diffraction measurements show that the crystal structure of the DPPE films did not change significantly upon exposure to water and surfactant solutions. I n contrast, FITC-DPPE films exhibited changes in the long-range spacing of their crystalline structure upon hydration.

Original languageEnglish (US)
Pages (from-to)247-251
Number of pages5
JournalThin Solid Films
Volume327-329
Issue number1-2
StatePublished - 1998
Externally publishedYes

Fingerprint

Phospholipids
Surface-Active Agents
Hydration
hydration
Surface active agents
surfactants
Critical micelle concentration
Water
micelles
Salts
water
Crystalline materials
salts
Molecular Probes
X ray diffraction
Anionic surfactants
Quartz crystal microbalances
Sodium dodecyl sulfate
quartz crystals
sodium sulfates

Keywords

  • Hydration
  • Phospholipid
  • Quartz crystal microbalance
  • Surfactant

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Condensed Matter Physics
  • Surfaces and Interfaces

Cite this

Colberg, M. T., Carnes, K., Saez, A. E., Grant, C. S., Hutchinson, K., & Hesterberg, D. (1998). Hydration and removal of supported phospholipid films in aqueous surfactant solutions. Thin Solid Films, 327-329(1-2), 247-251.

Hydration and removal of supported phospholipid films in aqueous surfactant solutions. / Colberg, M. T.; Carnes, K.; Saez, Avelino E; Grant, C. S.; Hutchinson, K.; Hesterberg, D.

In: Thin Solid Films, Vol. 327-329, No. 1-2, 1998, p. 247-251.

Research output: Contribution to journalArticle

Colberg, MT, Carnes, K, Saez, AE, Grant, CS, Hutchinson, K & Hesterberg, D 1998, 'Hydration and removal of supported phospholipid films in aqueous surfactant solutions', Thin Solid Films, vol. 327-329, no. 1-2, pp. 247-251.
Colberg MT, Carnes K, Saez AE, Grant CS, Hutchinson K, Hesterberg D. Hydration and removal of supported phospholipid films in aqueous surfactant solutions. Thin Solid Films. 1998;327-329(1-2):247-251.
Colberg, M. T. ; Carnes, K. ; Saez, Avelino E ; Grant, C. S. ; Hutchinson, K. ; Hesterberg, D. / Hydration and removal of supported phospholipid films in aqueous surfactant solutions. In: Thin Solid Films. 1998 ; Vol. 327-329, No. 1-2. pp. 247-251.
@article{abfa837602e44c67a2f9578d69a8fc55,
title = "Hydration and removal of supported phospholipid films in aqueous surfactant solutions",
abstract = "Dynamic studies of the hydration and removal of phospholipid films attached to solid substrates were performed. The phospholipids used were 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine (DPPE), and a modification of DPPE containing a fluorescent molecular probe: n-(5-fluoresceinthicarbamoyl)-1,2-dipalmitoyl-sn-glycero-3- phosphatidylethanolamine, triethylammonium salt (FITC-DPPE). The phospholipid films were exposed to water and aqueous solutions of the anionic surfactant sodium dodecyl sulfate (SDS). The film mass was determined as a function of time by means of a quartz crystal microbalance (QCM). The crystalline structure of the film during the hydration process was analyzed by means of wide-angle X-ray diffraction. At low surfactant concentrations (below 20{\%} of the critical micelle concentration (CMC)), the presence of surfactant increased the hydration rate of the film, as well as its maximum water uptake. At surfactant concentrations as low as 50{\%} of the CMC, competitive hydration and removal of the phospholipid film were observed. X-Ray diffraction measurements show that the crystal structure of the DPPE films did not change significantly upon exposure to water and surfactant solutions. I n contrast, FITC-DPPE films exhibited changes in the long-range spacing of their crystalline structure upon hydration.",
keywords = "Hydration, Phospholipid, Quartz crystal microbalance, Surfactant",
author = "Colberg, {M. T.} and K. Carnes and Saez, {Avelino E} and Grant, {C. S.} and K. Hutchinson and D. Hesterberg",
year = "1998",
language = "English (US)",
volume = "327-329",
pages = "247--251",
journal = "Thin Solid Films",
issn = "0040-6090",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Hydration and removal of supported phospholipid films in aqueous surfactant solutions

AU - Colberg, M. T.

AU - Carnes, K.

AU - Saez, Avelino E

AU - Grant, C. S.

AU - Hutchinson, K.

AU - Hesterberg, D.

PY - 1998

Y1 - 1998

N2 - Dynamic studies of the hydration and removal of phospholipid films attached to solid substrates were performed. The phospholipids used were 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine (DPPE), and a modification of DPPE containing a fluorescent molecular probe: n-(5-fluoresceinthicarbamoyl)-1,2-dipalmitoyl-sn-glycero-3- phosphatidylethanolamine, triethylammonium salt (FITC-DPPE). The phospholipid films were exposed to water and aqueous solutions of the anionic surfactant sodium dodecyl sulfate (SDS). The film mass was determined as a function of time by means of a quartz crystal microbalance (QCM). The crystalline structure of the film during the hydration process was analyzed by means of wide-angle X-ray diffraction. At low surfactant concentrations (below 20% of the critical micelle concentration (CMC)), the presence of surfactant increased the hydration rate of the film, as well as its maximum water uptake. At surfactant concentrations as low as 50% of the CMC, competitive hydration and removal of the phospholipid film were observed. X-Ray diffraction measurements show that the crystal structure of the DPPE films did not change significantly upon exposure to water and surfactant solutions. I n contrast, FITC-DPPE films exhibited changes in the long-range spacing of their crystalline structure upon hydration.

AB - Dynamic studies of the hydration and removal of phospholipid films attached to solid substrates were performed. The phospholipids used were 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine (DPPE), and a modification of DPPE containing a fluorescent molecular probe: n-(5-fluoresceinthicarbamoyl)-1,2-dipalmitoyl-sn-glycero-3- phosphatidylethanolamine, triethylammonium salt (FITC-DPPE). The phospholipid films were exposed to water and aqueous solutions of the anionic surfactant sodium dodecyl sulfate (SDS). The film mass was determined as a function of time by means of a quartz crystal microbalance (QCM). The crystalline structure of the film during the hydration process was analyzed by means of wide-angle X-ray diffraction. At low surfactant concentrations (below 20% of the critical micelle concentration (CMC)), the presence of surfactant increased the hydration rate of the film, as well as its maximum water uptake. At surfactant concentrations as low as 50% of the CMC, competitive hydration and removal of the phospholipid film were observed. X-Ray diffraction measurements show that the crystal structure of the DPPE films did not change significantly upon exposure to water and surfactant solutions. I n contrast, FITC-DPPE films exhibited changes in the long-range spacing of their crystalline structure upon hydration.

KW - Hydration

KW - Phospholipid

KW - Quartz crystal microbalance

KW - Surfactant

UR - http://www.scopus.com/inward/record.url?scp=0032141167&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032141167&partnerID=8YFLogxK

M3 - Article

VL - 327-329

SP - 247

EP - 251

JO - Thin Solid Films

JF - Thin Solid Films

SN - 0040-6090

IS - 1-2

ER -