Hydraulic approach in the scale up of the ebb-and-flow bioreactor (EFBR) for `hairy root' cultures

Chun Yue, Joel L. Cuello, Kenneth A. Jordan

Research output: Contribution to journalConference article

Abstract

An ebb-and-flow bioreactor (EFBR) hold-up simulation model for `hairy' roots was earlier proposed by Cuello (1994). In this study, by experimentally determining the water characteristic curve for Hyoscyamus muticus `hairy root' culture, the proposed model was implemented and was validated using a segmented EFBR (SEFBR). The water characteristic curve was determined at an average porosity of 88%, whereas the SEFBR had an average porosity of 77%. The simulated dynamic average hold-up profiles and the simulated dynamic spatial hold-up distribution produced generally acceptable error of less than 10%.

Original languageEnglish (US)
JournalPaper - American Society of Agricultural Engineers
Volume3
StatePublished - Dec 1 1997
EventProceedings of the 1997 ASAE Annual International Meeting. Part 1 (of 3) - Minneapolis, MN, USA
Duration: Aug 10 1997Aug 14 1997

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ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)

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